WB (Western Blot)
(Western blot analysis of RAD9 (Phospho-Ser272) using H2O2 treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit anti-Human RAD9 Polyclonal Antibody | anti-RAD9 antibody
Phospho-RAD9 (Ser272) Antibody
Gene Names
RAD9A; RAD9
Reactivity
Human
Applications
ELISA, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
RAD9, Antibody; Phospho-RAD9 (Ser272) Antibody; Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; Rad9a; RAD9A_HUMAN; anti-RAD9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-RAD9 (Ser272) Antibody detects endogenous levels of RAD9 only when phosphorylated at Ser272.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RAD9 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human RAD9 around the phosphorylation site of Ser272.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Subunit Structure
Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.
Similarity
Belongs to the rad9 family.
Subcellular Location
Nucleus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of RAD9 (Phospho-Ser272) using H2O2 treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of RAD9 (Phospho-Ser272) using H2O2 treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from Raji cells(serum starvation treatment), using Phospho-RAD9 (Ser272) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Raji cells(serum starvation treatment), using Phospho-RAD9 (Ser272) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-RAD9 antibody
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
391
NCBI Official Full Name
Cell cycle checkpoint control protein RAD9A
NCBI Official Synonym Full Names
RAD9 homolog A (S. pombe)
NCBI Official Symbol
RAD9A
NCBI Official Synonym Symbols
RAD9
NCBI Protein Information
cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A
UniProt Protein Name
Cell cycle checkpoint control protein RAD9A
UniProt Gene Name
RAD9A
UniProt Synonym Gene Names
hRAD9
UniProt Entry Name
RAD9A_HUMAN
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Product Notes
The RAD9 rad9a (Catalog #AAA329626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-RAD9 (Ser272) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAD9 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the RAD9 rad9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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