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IP (Immunoprecipitation)
(CRADD was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate1 uL anti-CRADD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CRADD rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
Rabbit anti-Human RAIDD Polyclonal Antibody | anti-CRADD antibody
Anti-RAIDD Antibody, Rabbit Polyclonal
Gene Names
CRADD; MRT34; RAIDD
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
RAIDD, Antibody; Anti-RAIDD Antibody, Rabbit Polyclonal; CRADD/RAIDD Antibody, Rabbit PAb, Antigen Affinity Purified; CASP2 and RIPK1 domain containing adaptor with death domain; Anti-MRT34 Antibody; Anti-RAIDD Antibody; anti-CRADD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CRADD/RAIDD
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CRADD antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human CRADD/RAIDD Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CRADD/RAIDD (rh CRADD/RAIDD). CRADD/RAIDD specific IgG was purified by Human CRADD/RAIDD affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CRADD was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate1 uL anti-CRADD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CRADD rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
IP (Immunoprecipitation)
(CRADD was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate1 uL anti-CRADD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CRADD rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of CRADD in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CRADD polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of CRADD in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CRADD polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining of human CRADD in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemisry)
(Immunochemical staining of human CRADD in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CRADD in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CRADD in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
WB (Western Blot)
(Anti-CRADD rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-CRADD rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CRADD antibody
Death domain-containing protein CRADD, also known as Caspase and RIP adapter with death domain, RIP-associated protein with a death domain, CRADD and RAIDD, is a protein which is constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. CRADD/RAIDD contains oneCARD domain and onedeath domain. CRADD/RAIDD contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2. FADD/MORT1 is a death domain (DD)-containing adaptor/signaling molecule that interacts with the intracellular DD of FAS/APO-I (CD95) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 (Mch5/MACH/FLICE). CRADD/RAIDD has a dual-domain structure similar to that of FADD. CRADD/RAIDD has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD/RAIDD could play a role in regulating apoptosis in mammalian cells. CRADD/RAIDD is a apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD/RAIDD recruits caspase-2 to the TNFR-1 signalling complex.
Product Categories/Family for anti-CRADD antibody
References
Ahmad, M. et al., 1997, Cancer Res. 57 (4):615-9. Chou J.J., et al., 1998, Cell 94:171-80. Tang, J. et al., 2005, Cancer Biol Ther. 4 (6):645-9. Vakifahmetoglu H., et al., 2006, Oncogene 25:5683-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
199
NCBI Official Full Name
Death domain-containing protein CRADD
NCBI Official Synonym Full Names
CASP2 and RIPK1 domain containing adaptor with death domain
NCBI Official Symbol
CRADD
NCBI Official Synonym Symbols
MRT34; RAIDD
NCBI Protein Information
death domain-containing protein CRADD; death adaptor molecule RAIDD; caspase and RIP adaptor with death domain; RIP-associated protein with a death domain; RIP-associated ICH1/CED3-homologous protein with death domain
UniProt Protein Name
Death domain-containing protein CRADD
UniProt Gene Name
CRADD
UniProt Synonym Gene Names
RAIDD
UniProt Entry Name
CRADD_HUMAN
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Product Notes
The CRADD cradd (Catalog #AAA255616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAIDD Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAIDD can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CRADD cradd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAIDD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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