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WB (Western Blot)
(Detection of human RALY by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, Hep-G2, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALY antibody (AAA211751 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RALY Polyclonal Antibody | anti-RALY antibody
Rabbit anti-RALY Antibody, Affinity Purified
Gene Names
RALY; P542; HNRPCL2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RALY, Antibody; Rabbit anti-RALY Antibody, Affinity Purified; hnRNP associated with lethal yellow protein homolog; RNA-binding protein Raly; RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow); RNA-binding protein (autoantigenic); RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog); P542; hnRNP core protein C-like 2; heterogeneous nuclear ribonucleoprotein C-like 2; autoantigen p542; HNRPCL2; RALY heterogeneous nuclear ribonucleoprotein; anti-RALY antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-RALY antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 256 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RALY by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, Hep-G2, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALY antibody (AAA211751 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human RALY by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, Hep-G2, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALY antibody (AAA211751 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RALY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RALY antibody (AAA211751 lot 2) used for IP at 6 ug per reaction. RALY was also immunoprecipitated by a previous lot of this antibody (AAA211751 lot 1) and a second antibody against a different epitope of RALY . For blotting immunoprecipitated RALY, AAA211751 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human RALY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RALY antibody (AAA211751 lot 2) used for IP at 6 ug per reaction. RALY was also immunoprecipitated by a previous lot of this antibody (AAA211751 lot 1) and a second antibody against a different epitope of RALY . For blotting immunoprecipitated RALY, AAA211751 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of mouse RALY by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RALY (AAA211751 lot 2) used at a dilution of 1:1,000 (0.2 ug/ml).)
IHC (Immunohiostchemistry)
(Detection of mouse RALY by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RALY (AAA211751 lot 2) used at a dilution of 1:1,000 (0.2 ug/ml).)
IHC (Immunohistochemistry)
(Detection of human RALY by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RALY (AAA211751 lot2) used at a dilution of 1:1,000 (0.2 ug/ml).)
IHC (Immunohistochemistry)
(Detection of human RALY by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RALY (AAA211751 lot2) used at a dilution of 1:1,000 (0.2 ug/ml).)
Related Product Information for anti-RALY antibody
The agouti (a) locus includes the mouse agouti gene that regulates coat color in mice by controlling the relative amount and distribution of yellow and black pigment in the coat hairs. A mutation in the agouti locus, lethal yellow (Ay), results in an all-yellow coat color along with a number of dominant pleiotropic effects such as obesity, diabetes, tumor susceptibility, and embryonic lethality in homozygotes. Molecular analysis of the Ay transcript revealed that the Ay transcript includes the coding region of the a gene but also included novel sequence. The novel sequence was found to originate from a 5'-noncoding exon of a gene that was named Raly, because it had the potential to encode an hnRNP protein that is associated with lethal yellow.
Product Categories/Family for anti-RALY antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,364 Da
NCBI Official Full Name
RNA-binding protein Raly isoform 1
NCBI Official Synonym Full Names
RALY heterogeneous nuclear ribonucleoprotein
NCBI Official Symbol
RALY
NCBI Official Synonym Symbols
P542; HNRPCL2
NCBI Protein Information
RNA-binding protein Raly
UniProt Protein Name
RNA-binding protein Raly
UniProt Gene Name
RALY
UniProt Synonym Gene Names
HNRPCL2; P542; hnRNP core protein C-like 2
UniProt Entry Name
RALY_HUMAN
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Product Notes
The RALY raly (Catalog #AAA211751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RALY Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RALY can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RALY raly for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RALY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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