WB (Western Blot)
(Detection of human RanBP1 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody AAA210992 Lot 3 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human RanBP1 Polyclonal Antibody | anti-RANBP1 antibody
Rabbit anti-RanBP1 Antibody, Affinity Purified
Gene Names
RANBP1; HTF9A; MGC88701; RANBP1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RanBP1, Antibody; Rabbit anti-RanBP1 Antibody, Affinity Purified; Ran-binding protein 1; testis secretory sperm-binding protein Li 221n; RanBP1; HTF9A; HpaII tiny fragments locus 9A; ran-specific GTPase-activating protein; Ran-specific GTPase-activating protein; RAN binding protein 1; anti-RANBP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RANBP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RanBP1 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody AAA210992 Lot 3 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human RanBP1 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody AAA210992 Lot 3 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RanBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP1 antibody AAA210992 Lot 3 used for IP at 6 ug per reaction. RanBP1 was also immunoprecipitated by a previous lot of this antibody (AAA210992 Lot 2) and rabbit anti-RanBP1 antibody For blotting immunoprecipitated RanBP1, AAA210992 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human RanBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP1 antibody AAA210992 Lot 3 used for IP at 6 ug per reaction. RanBP1 was also immunoprecipitated by a previous lot of this antibody (AAA210992 Lot 2) and rabbit anti-RanBP1 antibody For blotting immunoprecipitated RanBP1, AAA210992 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human RanBP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RanBP1 (AAA210992 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RanBP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RanBP1 (AAA210992 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-RANBP1 antibody
Ran-binding protein 1 (RanBP1) binds to the GTP-binding protein, Ran, when it is specifically complexed with GTP. RanBP1 binding to GTP-charged RAN increases the catalytic efficiency of GTP hydrolysis activated by RanGAP, the Ran specific GTPase-activating protein. RanBP1 is also a negative regulator of RCC1 which functions in the nuclear pore transport process and cell cycle progression.
Product Categories/Family for anti-RANBP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
ran-specific GTPase-activating protein [Homo sapiens]
NCBI Official Synonym Full Names
RAN binding protein 1
NCBI Official Symbol
RANBP1
NCBI Official Synonym Symbols
HTF9A; MGC88701; RANBP1
UniProt Protein Name
Ran-specific GTPase-activating protein
UniProt Gene Name
RANBP1
UniProt Synonym Gene Names
RanBP1
UniProt Entry Name
RANG_HUMAN
Similar Products
Product Notes
The RANBP1 ranbp1 (Catalog #AAA210992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RanBP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RanBP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RANBP1 ranbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RanBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.