WB (Western Blot)
(Detection of human and mouse RanBP2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody AAA211635 (lot AAA211635-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RanBP2 Polyclonal Antibody | anti-RanBP2 antibody
Rabbit anti-RanBP2 Antibody, Affinity Purified
Gene Names
RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RanBP2, Antibody; Rabbit anti-RanBP2 Antibody, Affinity Purified; nuclear pore complex protein Nup358; TRP1; transformation-related protein 2; RanBP2; ran-binding protein 2; P270; NUP358; TRP2; IIAE3; E3 SUMO-protein transferase RanBP2; E3 SUMO-protein ligase RanBP2; ANE1; ADANE; acute necrotizing encephalopathy 1 (autosomal dominant); 358 kDa nucleoporin; nucleoporin Nup358; nucleoporin 358; RAN binding protein 2; anti-RanBP2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-RanBP2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Chimpanzee
Immunogen
Between 1050 and 1100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RanBP2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody AAA211635 (lot AAA211635-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse RanBP2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody AAA211635 (lot AAA211635-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RanBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP2 antibody AAA211635 (lot AAA211635-3) used for IP at 6 ug per reaction. RanBP2 was also immunoprecipitated by a previous lot of this antibody (lot AAA211635-2) and rabbit anti-RanBP2 antibody For blotting immunoprecipitated RanBP2, AAA211635 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human RanBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP2 antibody AAA211635 (lot AAA211635-3) used for IP at 6 ug per reaction. RanBP2 was also immunoprecipitated by a previous lot of this antibody (lot AAA211635-2) and rabbit anti-RanBP2 antibody For blotting immunoprecipitated RanBP2, AAA211635 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-RanBP2 antibody
Ran binding protein 2 (RanBP2) is a large multi-modular nucleoporin protein (also called NUP358) that bears several structural domains including a cyclophilin-type rotamase domain, four RanBD1 domains, eight RanBP2-type zinc fingers, and one TPR repeat. This variety of structural domains imparts pleiotropic qualities to RanBP2. RanBP2 has been shown to function as an E3 SUMO-protein ligase and is also part of the nuclear export pathway.
Product Categories/Family for anti-RanBP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358,199 Da
NCBI Official Full Name
E3 SUMO-protein ligase RanBP2
NCBI Official Synonym Full Names
RAN binding protein 2
NCBI Official Symbol
RANBP2
NCBI Official Synonym Symbols
ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
NCBI Protein Information
E3 SUMO-protein ligase RanBP2; P270; nucleoporin 358; nucleoporin Nup358; 358 kDa nucleoporin; ran-binding protein 2; transformation-related protein 2; nuclear pore complex protein Nup358; acute necrotizing encephalopathy 1 (autosomal dominant)
UniProt Protein Name
E3 SUMO-protein ligase RanBP2
UniProt Gene Name
RANBP2
UniProt Synonym Gene Names
NUP358; RanBP2
UniProt Entry Name
RBP2_HUMAN
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Product Notes
The RanBP2 ranbp2 (Catalog #AAA211635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RanBP2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RanBP2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RanBP2 ranbp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RanBP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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