WB (Western Blot)
(Detection of human RAP80 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP80 antibody AAA211127 (lot AAA211127-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human RAP80 Polyclonal Antibody | anti-RAP80 antibody
Rabbit anti-RAP80 Antibody, Affinity Purified
Gene Names
UIMC1; RAP80; X2HRIP110
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RAP80, Antibody; Rabbit anti-RAP80 Antibody, Affinity Purified; RAP80; X2HRIP110; Ubiquitin interaction motif-containing protein 1; BRCA1-A complex subunit RAP80; receptor-associated protein 80; retinoid X receptor-interacting protein 110; ubiquitin interaction motif containing 1; anti-RAP80 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RAP80 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 669 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RAP80 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP80 antibody AAA211127 (lot AAA211127-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human RAP80 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP80 antibody AAA211127 (lot AAA211127-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human RAP80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RAP80 antibody AAA211127 (lot AAA211127-2) used for IP at 6 ug per reaction. RAP80 was also immunoprecipitated by a previous lot of this antibody (lot AAA211127-1) and rabbit anti-RAP80 antibody For blotting immunoprecipitated RAP80, AAA211127 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RAP80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RAP80 antibody AAA211127 (lot AAA211127-2) used for IP at 6 ug per reaction. RAP80 was also immunoprecipitated by a previous lot of this antibody (lot AAA211127-1) and rabbit anti-RAP80 antibody For blotting immunoprecipitated RAP80, AAA211127 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human RAP80 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAP80 (Cat. No. AAA211127 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RAP80 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAP80 (Cat. No. AAA211127 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-RAP80 antibody
RAP80 was first identified as a nuclear protein that interacts with the retinoid-related testis-associated receptor (RTR) and is able to inhibit the interaction of RTR with the N-CoR co-repressor. RAP80 has also been shown to interact with estrogen receptor alpha (ER-alpha) and modulate its activity. The modulation of ER-alpha activity is dependent on the ubiquitin-interacting motif (UIM) of RAP80. The UIM of RAP80 has also been demonstrated to be important for the BRCA1-dependent DNA repair response.
Product Categories/Family for anti-RAP80 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,412 Da
NCBI Official Full Name
BRCA1-A complex subunit RAP80
NCBI Official Synonym Full Names
ubiquitin interaction motif containing 1
NCBI Official Symbol
UIMC1
NCBI Official Synonym Symbols
RAP80; X2HRIP110
NCBI Protein Information
BRCA1-A complex subunit RAP80; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1
UniProt Protein Name
BRCA1-A complex subunit RAP80
UniProt Gene Name
UIMC1
UniProt Synonym Gene Names
RAP80; RXRIP110
UniProt Entry Name
UIMC1_HUMAN
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Product Notes
The RAP80 uimc1 (Catalog #AAA211127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RAP80 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP80 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RAP80 uimc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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