Rabbit anti-Human, mouse RARA Polyclonal Antibody | anti-RARA antibody

RARA Antibody (C-term)

Cat. Num. AAA289217

• Gene Names: RARA; RAR; NR1B1
• Reactivity: Human, mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RARA, Antibody; RARA Antibody (C-term); Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1; RARA; NR1B1; anti-RARA antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.31mg/ml (varies by lot)
• Sequence Positions: 322-349
• Sequence Length: 349

• Applicable Applications for anti-RARA antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, IF (Immunofluorescence)
• Cellular Location: Nucleus. Cytoplasm. Note=Nuclearlocalization depends on ligand binding,phosphorylation and sumoylation(PubMed:19850744) Translocation to thenucleus in the absence of ligand is dependenton activation of PKC and the downstreamMAPK phosphorylation (By similarity).Increased nuclear localization upon pulsatileshear stress (PubMed:28167758).{ECO:0000250|UniProtKB:P11416,ECO:0000269|PubMed:19850744,ECO:0000269|PubMed:28167758}
• Tissue Location: Expressed in monocytes.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(RARA Antibody (C-term)(AAA289217) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(RARA Antibody (C-term) (AAA289217) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of RARA Antibody (C-term) (AAA289217) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(All lanes : Anti-RARA Antibody (C-term) at1:1000 dilution Lane 1: HL-60 whole cell lysateLane 2: MCF-7 whole cell lysate Lane 3: HepG2whole cell lysate Lane 4: Hela whole cell lysateLane 5: T47D whole cell lysate Lysates/proteinsat 20 ?g per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(RARA Antibody (C-term) (AAA289217) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow).)

Related Product Information for anti-RARA antibody

Background: This gene represents a nuclear retinoic acidreceptor. The encoded protein, retinoic acid receptor alpha,regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively splicedtranscript variants have been found for this locus.

Function: Receptor for retinoic acid (PubMed: 19850744, PubMed: 16417524,PubMed: 20215566). Retinoicacid receptors bind as heterodimers to their target response elements in response to theirligands, all-trans or 9- cis retinoic acid, andregulate gene expression in various biologicalprocesses (PubMed: 28167758). TheRXR/RAR heterodimers bind to the retinoicacid response elements (RARE) composed oftandem 5'-AGGTCA-3' sites known asDR1-DR5 (PubMed: 28167758). In theabsence of ligand, the RXR-RAR heterodimers associate with a multiprotein complexc ontaining transcription corepressors that induce histone deacetylation, chromatincondensation and transcriptional suppression (PubMed: 16417524). On ligandbinding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation (PubMed: 9267036, PubMed: 19850744,PubMed: 20215566). Formation of a complex with histone deacetylases mightlead to inhibition of RARE DNA elementbinding and to transcriptional repression(PubMed: 28167758).Transcriptional activation and RARE DNAelement binding might be supported by the transcription factor KLF2 (PubMed: 28167758). RARA plays an essential role in the regulation ofretinoic acid-induced germ cell development during spermatogenesis (By similarity). Has arole in the survival of early spermatocytes atthe beginning prophase of meiosis (By similarity). In Sertoli cells, may promote thesurvival and development of early meioticprophase spermatocytes (By similarity). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Together with RXRA,positively regulates microRNA-10a expression, thereby inhibiting theGATA6/VCAM1 signaling response to pulsatileshear stress in vascular endothelial cells RARA Antibody (C-term) (MBS9212799) western blot analysis in HL-60 cell line andmouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow). RARA Antibody (C-term) (MBS9212799)immunohistochemistry analysis informalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. (PubMed: 28167758). Inassociation with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and there by promotes the inflammatory response (PubMed: 28167758).

Product Categories/Family for anti-RARA antibody

Crown Antibodies; Cancer; Signal Transduction

References

Park, U.H., et al. J. Biol. Chem. 285(44):34269-34278(2010)
Asleson, A.D., et al. Cancer Genet. Cytogenet. 202(1):33-37(2010)
Catalano, A., et al. Blood 110(12):4073-4076(2007)
Wells, R.A., et al. Nat. Genet. 17(1):109-113(1997)
Chen, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 91(3):1178-1182(1994)

NCBI and Uniprot Product Information

• NCBI GI #: 4506419
• NCBI GeneID: 5914
• NCBI Accession #: NP_000955.1; NP_001019980.1; NP_001138773.1; NP_001138774.1
• NCBI GenBank Nucleotide #: NM_000964.3
• UniProt Accession #: P10276; P78456; Q13440; Q13441; Q96S41; Q9NQS0; B8Y636
• Molecular Weight: 50771
• NCBI Official Full Name: retinoic acid receptor alpha isoform 1
• NCBI Official Synonym Full Names: retinoic acid receptor, alpha
• NCBI Official Symbol: RARA
• NCBI Official Synonym Symbols: RAR; NR1B1
• NCBI Protein Information: retinoic acid receptor alpha
• UniProt Protein Name: Retinoic acid receptor alpha
• UniProt Gene Name: RARA
• UniProt Synonym Gene Names: NR1B1; RAR-alpha
• UniProt Entry Name: RARA_HUMAN