IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with RARB Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.)
Rabbit RARB Polyclonal Antibody | anti-RARB antibody
RARB Antibody (Center)
Gene Names
RARB; HAP; RRB2; NR1B2; MCOPS12
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RARB, Antibody; RARB Antibody (Center); Retinoic acid receptor beta; RAR-beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon; RARB; HAP; NR1B2; anti-RARB antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the Central region of human RARB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
168-195
Sequence Length
–
Applicable Applications for anti-RARB antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with RARB Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with RARB Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.)
WB (Western Blot)
(RARB Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RARB antibody detected the RARB protein (arrow).)
WB (Western Blot)
(RARB Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RARB antibody detected the RARB protein (arrow).)
Related Product Information for anti-RARB antibody
This gene encodes retinoic acid receptor beta, a member of
the thyroid-steroid hormone receptor superfamily of nuclear
transcriptional regulators. This receptor localizes to the
cytoplasm and to subnuclear compartments. It binds retinoic acid,
the biologically active form of vitamin A which mediates cellular
signalling in embryonic morphogenesis, cell growth and
differentiation. It is thought that this protein limits growth of
many cell types by regulating gene expression. The gene was first
identified in a hepatocellular carcinoma where it flanks a
hepatitis B virus integration site. The gene expresses at least two
transcript variants; one additional transcript has been described,
but its full length nature has not been determined. [provided by
RefSeq].
the thyroid-steroid hormone receptor superfamily of nuclear
transcriptional regulators. This receptor localizes to the
cytoplasm and to subnuclear compartments. It binds retinoic acid,
the biologically active form of vitamin A which mediates cellular
signalling in embryonic morphogenesis, cell growth and
differentiation. It is thought that this protein limits growth of
many cell types by regulating gene expression. The gene was first
identified in a hepatocellular carcinoma where it flanks a
hepatitis B virus integration site. The gene expresses at least two
transcript variants; one additional transcript has been described,
but its full length nature has not been determined. [provided by
RefSeq].
Product Categories/Family for anti-RARB antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Ding, Y., et al. Mol. Vis. 16, 855-861 (2010) :
Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Ding, Y., et al. Mol. Vis. 16, 855-861 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50489
NCBI Official Full Name
retinoic acid receptor beta isoform 1
NCBI Official Synonym Full Names
retinoic acid receptor, beta
NCBI Official Symbol
RARB
NCBI Official Synonym Symbols
HAP; RRB2; NR1B2; MCOPS12
NCBI Protein Information
retinoic acid receptor beta
UniProt Protein Name
Retinoic acid receptor beta
UniProt Gene Name
RARB
UniProt Synonym Gene Names
HAP; NR1B2; RAR-beta
UniProt Entry Name
RARB_HUMAN
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Product Notes
The RARB rarb (Catalog #AAA285584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 168-195. The RARB Antibody (Center) reacts with Human (Predicted Reactivity: Chicken, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RARB can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RARB rarb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RARB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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