Rabbit anti-Human, Mouse RbBP5 Polyclonal Antibody | anti-RbBP5 antibody

Rabbit anti-RbBP5 Antibody, Affinity Purified

Cat. Num. AAA23769

• Gene Names: RBBP5; RBQ3; SWD1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: RbBP5; Polyclonal Antibody; Rabbit anti-RbBP5 Antibody; Affinity Purified; SWD1; RBQ3; Set1c WD40 repeat protein; homolog; retinoblastoma-binding protein 5; RBBP-5; retinoblastoma-binding protein RBQ-3; Retinoblastoma-binding protein 5; RB binding protein 5; histone lysine methyltransferase complex subunit; anti-RbBP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-RbBP5 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ChImmunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq)
• Application Notes: WB: 1:10,000-1:25,000; IP: 6ug/mg lysate; IHC: 1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:250-1:1000; ChIP: 1-3 ug as per Dou et al., Nat Struct Mol Biol 13 (8):713-719, 2006. Previous lots of this antibody have performed in this application.; ChIP-chip: 10 ug. Previous lots of this antibody have performed in this application.; ChIP-Seq: 2 ug. Previous lots of this antibody have performed in this application.
• Immunogen: Between 488 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse RbBP5 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody (AAA23769 lot 5) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

WB (Western Blot)

(Detection of human RbBP5 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, NCI-H460, HEK293T, Malme-3M, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody (AAA23769 lot 5) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation)

(Detection of human RbBP5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RbBP5 antibody (AAA23769 lot 5) used for IP at 6 ug per reaction. RbBP5 was also immunoprecipitated by a previous lot of this antibody (AAA23769 lot 4). For blotting immunoprecipitated RbBP5, AAA23769 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human RbBP5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 (AAA23769 lot 5) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

ChIP (Chromatin Immunoprecipitation)

(Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody AAA23769 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody AAA23769. ChIP-seq validation performed by Diogenode, Denville, NJ.)

ChIP (Chromatin Immunoprecipitation)

(ChIP-chip scatter plot of anti-RbBP5 (AAA23769) enriched DNA binding sites versus input reference DNA. A. 10 ug of AAA23769 was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.)

Related Product Information for anti-RbBP5 antibody

Retinoblastoma binding protein 5 (RbBP5) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. RbBP5 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, ASH2L,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX. RbBP5 is also one of many proteins that bind the Retinoblastoma tumor suppressor protein, pRb.

Product Categories/Family for anti-RbBP5 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 53759148
• NCBI GeneID: 5929
• NCBI Accession #: NP_005048.2
• NCBI GenBank Nucleotide #: NM_005057.3
• UniProt Accession #: Q15291; Q7Z6D8; Q8NDZ7; A8K272
• Molecular Weight: 59,153 Da
• NCBI Official Full Name: retinoblastoma-binding protein 5 isoform 1
• NCBI Official Synonym Full Names: retinoblastoma binding protein 5
• NCBI Official Symbol: RBBP5
• NCBI Official Synonym Symbols: RBQ3; SWD1
• NCBI Protein Information: retinoblastoma-binding protein 5; RBBP-5; OTTHUMP00000035177; OTTHUMP00000035178; retinoblastoma-binding protein RBQ-3; SWD1, Set1c WD40 repeat protein, homolog
• UniProt Protein Name: Retinoblastoma-binding protein 5
• UniProt Gene Name: RBBP5
• UniProt Synonym Gene Names: RBQ3; RBBP-5
• UniProt Entry Name: RBBP5_HUMAN