FCM/FACS (Flow Cytometry)
(RBBP7 Antibody (N-term) (AAA287297) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit RBBP7 Polyclonal Antibody | anti-RBBP7 antibody
RBBP7 Antibody (N-term)
Gene Names
RBBP7; RbAp46
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat, Xenopus)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RBBP7, Antibody; RBBP7 Antibody (N-term); Histone-binding protein RBBP7; Histone acetyltransferase type B subunit 2; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7; Retinoblastoma-binding protein p46; RBBP7; RBAP46; anti-RBBP7 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This RBBP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RBBP7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
1-30
Sequence Length
469
Applicable Applications for anti-RBBP7 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Cellular Location
Nucleus
Function
Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex
Preparation and Storage
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RBBP7 Antibody (N-term) (AAA287297) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RBBP7 Antibody (N-term) (AAA287297) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of RBBP7 Antibody (N-term) (AAA287297) in Hela, MDA-MB231, 293 cell line lysates (35ug/lane). RBBP7 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of RBBP7 Antibody (N-term) (AAA287297) in Hela, MDA-MB231, 293 cell line lysates (35ug/lane). RBBP7 (arrow) was detected using the purified Pab.)
Related Product Information for anti-RBBP7 antibody
RBBP7 is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. This protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation.
References
Zhang,Y., et.al., Mol. Cell 1 (7), 1021-1031 (1998)
Verreault,A., et.al., Curr. Biol. 8 (2), 96-108 (1998)
Verreault,A., et.al., Curr. Biol. 8 (2), 96-108 (1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47820
NCBI Official Full Name
histone-binding protein RBBP7 isoform 1
NCBI Official Synonym Full Names
retinoblastoma binding protein 7
NCBI Official Symbol
RBBP7
NCBI Official Synonym Symbols
RbAp46
NCBI Protein Information
histone-binding protein RBBP7
UniProt Protein Name
Histone-binding protein RBBP7
UniProt Gene Name
RBBP7
UniProt Synonym Gene Names
RBAP46; RBBP-7
UniProt Entry Name
RBBP7_HUMAN
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Product Notes
The RBBP7 rbbp7 (Catalog #AAA287297) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The RBBP7 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's RBBP7 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RBBP7 rbbp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBBP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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