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IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with RBPJ Antibody (N-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.)
Rabbit anti-Human RBPJ Polyclonal Antibody | anti-RBPJ antibody
RBPJ Antibody (N-term)
Gene Names
RBPJ; SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RBPJ, Antibody; RBPJ Antibody (N-term); Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30; RBPJ; IGKJRB; IGKJRB1; RBPJK; RBPSUH; anti-RBPJ antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RBPJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human RBPJ.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-29
Sequence Length
487
Applicable Applications for anti-RBPJ antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with RBPJ Antibody (N-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with RBPJ Antibody (N-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.)
IHC (Immunohistochemisry)
(RBPJ Antibody (N-term) (AAA285596)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(RBPJ Antibody (N-term) (AAA285596)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(RBPJ Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).)
WB (Western Blot)
(RBPJ Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).)
WB (Western Blot)
(Anti-RBPJ Antibody (N-term)at 1:2000 dilution + human pancreas lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-RBPJ Antibody (N-term)at 1:2000 dilution + human pancreas lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RBPJ antibody
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate. determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
Product Categories/Family for anti-RBPJ antibody
References
Chari, S., et al. J. Immunol. 185(1):410-417(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Stahl, E.A., et al. Nat. Genet. 42(6):508-514(2010)
Johnson, S.E., et al. J. Biol. Chem. 285(9):6681-6692(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Stahl, E.A., et al. Nat. Genet. 42(6):508-514(2010)
Johnson, S.E., et al. J. Biol. Chem. 285(9):6681-6692(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55637
NCBI Official Full Name
recombining binding protein suppressor of hairless isoform 1
NCBI Official Synonym Full Names
recombination signal binding protein for immunoglobulin kappa J region
NCBI Official Symbol
RBPJ
NCBI Official Synonym Symbols
SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
NCBI Protein Information
recombining binding protein suppressor of hairless
UniProt Protein Name
Recombining binding protein suppressor of hairless
UniProt Gene Name
RBPJ
UniProt Synonym Gene Names
IGKJRB; IGKJRB1; RBPJK; RBPSUH; RBP-J; RBP-JK
UniProt Entry Name
SUH_HUMAN
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Product Notes
The RBPJ rbpj (Catalog #AAA285596) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-29. The RBPJ Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBPJ can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RBPJ rbpj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBPJ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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