WB (Western Blot)
(Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL) [50 ug for WB; 1mg for IP, 20% of IP loaded] from HeLa cells. Lysate was prepared from untreated (-) cells or cells treated (+) with CoCl2. Antibodies: Mouse monoclonal anti-REDD1 antibody [1G11] was used at 1:1000 for WB and 3 ul/mg of lysate for IP. REDD1 was also immunoprecipitated (lanes 5&6) by rabbit anti-REDD1 antibody (AAA211789). Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human REDD1 Polyclonal Antibody | anti-REDD1 antibody
Rabbit anti-REDD1 Antibody, Affinity Purified
Gene Names
DDIT4; Dig2; REDD1; REDD-1
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
REDD1, Antibody; Rabbit anti-REDD1 Antibody, Affinity Purified; protein regulated in development and DNA damage response 1; REDD1; HIF-1 responsive protein RTP801; DNA damage-inducible transcript 4 protein; Dig2; REDD-1; DNA damage inducible transcript 4; anti-REDD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-REDD1 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Bovine, Dog, Horse, Rabbit, Panda, Orangutan, Monkey, Gorilla, Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL) [50 ug for WB; 1mg for IP, 20% of IP loaded] from HeLa cells. Lysate was prepared from untreated (-) cells or cells treated (+) with CoCl2. Antibodies: Mouse monoclonal anti-REDD1 antibody [1G11] was used at 1:1000 for WB and 3 ul/mg of lysate for IP. REDD1 was also immunoprecipitated (lanes 5&6) by rabbit anti-REDD1 antibody (AAA211789). Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL) [50 ug for WB; 1mg for IP, 20% of IP loaded] from HeLa cells. Lysate was prepared from untreated (-) cells or cells treated (+) with CoCl2. Antibodies: Mouse monoclonal anti-REDD1 antibody [1G11] was used at 1:1000 for WB and 3 ul/mg of lysate for IP. REDD1 was also immunoprecipitated (lanes 5&6) by rabbit anti-REDD1 antibody (AAA211789). Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
ICC (Immunocytochemistry)
(Detection of human REDD1 by immunocytochemistry. Sample: NBF-fixed asynchronous, Cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 (Cat. No. AAA211789 Lot1) used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight594 used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human REDD1 by immunocytochemistry. Sample: NBF-fixed asynchronous, Cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 (Cat. No. AAA211789 Lot1) used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight594 used at a dilution of 1:100.)
Related Product Information for anti-REDD1 antibody
REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner. Alternative names for REDD1 include DDIT4, DNA-damage-inducible transcript 4 protein, and RTP801.
Product Categories/Family for anti-REDD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,371 Da
NCBI Official Full Name
DNA damage-inducible transcript 4 protein
NCBI Official Synonym Full Names
DNA-damage-inducible transcript 4
NCBI Official Symbol
DDIT4
NCBI Official Synonym Symbols
Dig2; REDD1; REDD-1
NCBI Protein Information
DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1
UniProt Protein Name
DNA damage-inducible transcript 4 protein
UniProt Gene Name
DDIT4
UniProt Synonym Gene Names
REDD1; RTP801; REDD-1
UniProt Entry Name
DDIT4_HUMAN
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Product Notes
The REDD1 ddit4 (Catalog #AAA211789) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-REDD1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's REDD1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the REDD1 ddit4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REDD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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