WB (Western Blot)
(Detection of human RFC1 by western blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RFC1 antibody AAA210786 (lot AAA210786-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Goat anti-Human RFC1 Polyclonal Antibody | anti-RFC1 antibody
Goat anti-RFC1 Antibody, Affinity Purified
Gene Names
RFC1; A1; RFC; PO-GA; RECC1; MHCBFB; RFC140; MGC51786
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RFC1, Antibody; Goat anti-RFC1 Antibody, Affinity Purified; activator 1 140 kDa subunit; replication factor C (activator 1) 1, 145kDa; RFC140; RF-C 140 kDa subunit; RFC; replication factor C1; replication factor C subunit 1; replication factor C large subunit; replication factor C 140 kDa subunit; RECC1; PO-GA; MHCBFB; MHC binding factor, beta; DNA-binding protein PO-GA; activator 1 large subunit; A1 140 kDa subunit; A1; activator 1 subunit 1; Replication factor C subunit 1; anti-RFC1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RFC1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1098 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RFC1 by western blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RFC1 antibody AAA210786 (lot AAA210786-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human RFC1 by western blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RFC1 antibody AAA210786 (lot AAA210786-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RFC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC1 antibody AAA210786 (lot AAA210786-2) used for IP at 6 ug per reaction. RFC1 was also immunoprecipitated by rabbit anti-RFC1 antibody For blotting immunoprecipitated RFC1, AAA210786 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RFC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC1 antibody AAA210786 (lot AAA210786-2) used for IP at 6 ug per reaction. RFC1 was also immunoprecipitated by rabbit anti-RFC1 antibody For blotting immunoprecipitated RFC1, AAA210786 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-RFC1 antibody
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC1 is the 140 kDa subunit of RFC that has a DNA-binding domain with a BRCT motif and is suggested to play a more critical role than the other subunits.
Product Categories/Family for anti-RFC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128,255 Da
NCBI Official Full Name
replication factor C subunit 1 isoform 1
NCBI Official Synonym Full Names
replication factor C (activator 1) 1, 145kDa
NCBI Official Symbol
RFC1
NCBI Official Synonym Symbols
A1; RFC; PO-GA; RECC1; MHCBFB; RFC140; MGC51786
NCBI Protein Information
replication factor C subunit 1; A1 140 kDa subunit; OTTHUMP00000125201; OTTHUMP00000218411; RF-C 140 kDa subunit; activator 1 subunit 1; replication factor C1; MHC binding factor, beta; DNA-binding protein PO-GA; activator 1 large subunit; activator 1 140
UniProt Protein Name
Replication factor C subunit 1
UniProt Gene Name
RFC1
UniProt Synonym Gene Names
RFC140; A1 140 kDa subunit; RF-C 140 kDa subunit; RFC140
UniProt Entry Name
RFC1_HUMAN
Similar Products
Product Notes
The RFC1 rfc1 (Catalog #AAA210786) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RFC1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RFC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RFC1 rfc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.