ICC (Immunocytochemistry)
(Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.)
Rabbit RNF121 Polyclonal Antibody | anti-RNF121 antibody
RNF121 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
RNF121, Antibody; RNF121 Antibody; Ring finger protein 121; anti-RNF121 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
327
Applicable Applications for anti-RNF121 antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
MCF7 Cell Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
ICC (Immunocytochemistry)
(Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.)
ICC (Immunocytochemistry)
(Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.)
WB (Western Blot)
(Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot)
(Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-RNF121 antibody
RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).
Product Categories/Family for anti-RNF121 antibody
References
Darom A, Bening-Abu-Shach U, and Broday L. RNF-121 is an endoplasmic reticulum-membrane E3 ubiquitin ligase involved in the regulation of B-integrin. Mol. Biol. Cell 2010; 21:1788-98.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 24, 27, 36 kDa
Observed: 28 kDa
Observed: 28 kDa
NCBI Official Full Name
RING finger protein 121 isoform a
NCBI Official Synonym Full Names
ring finger protein 121
NCBI Official Symbol
RNF121
NCBI Protein Information
RING finger protein 121
UniProt Protein Name
RING finger protein 121
UniProt Gene Name
RNF121
UniProt Entry Name
RN121_HUMAN
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Product Notes
The RNF121 rnf121 (Catalog #AAA40850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF121 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF121 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RNF121 rnf121 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF121, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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