IF (Immunofluorescence)
(issue/cell: bone of mouse embryo;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(bs-9158R) 1:200, overnight at 4?C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37?C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
Rabbit anti-Rat, Cow RNF13 Polyclonal Antibody | anti-RNF13 antibody
RNF13 Polyclonal Antibody
Gene Names
RNF13; RZF
Reactivity
Rat, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF13, Antibody; RNF13 Polyclonal Antibody; E3 ubiquitin-protein ligase RNF13; 2.3.2.27; RING finger protein 13; RING-type E3 ubiquitin transferase RNF13; RNF13; RZF; anti-RNF13 antibody
Host
Rabbit
Reactivity
Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-RNF13 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(issue/cell: bone of mouse embryo;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(bs-9158R) 1:200, overnight at 4?C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37?C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IF (Immunofluorescence)
(issue/cell: bone of mouse embryo;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(bs-9158R) 1:200, overnight at 4?C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37?C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IHC (Immunohistochemistry)
(Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(bs-9158R) 1:200, overnight at 4?C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemistry)
(Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(bs-9158R) 1:200, overnight at 4?C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,713 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF13
NCBI Official Synonym Full Names
ring finger protein 13
NCBI Official Symbol
RNF13
NCBI Official Synonym Symbols
RZF
NCBI Protein Information
E3 ubiquitin-protein ligase RNF13; RING zinc finger protein
UniProt Protein Name
E3 ubiquitin-protein ligase RNF13
UniProt Gene Name
RNF13
UniProt Synonym Gene Names
RZF
UniProt Entry Name
RNF13_HUMAN
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Product Notes
The RNF13 rnf13 (Catalog #AAA292177) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF13 Polyclonal Antibody reacts with Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RNF13 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the RNF13 rnf13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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