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WB (Western Blot)
(Figure 1. Western blot analysis of RNF168 using anti-RNF168 antibody.)
Rabbit anti-Human RNF168 Polyclonal Antibody | anti-RNF168 antibody
Anti-RNF168 Antibody
Gene Names
RNF168; RIDL; hRNF168
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
RNF168, Antibody; Anti-RNF168 Antibody; E3 ubiquitin-protein ligase RNF168; hRNF168; RING finger protein 168; RING-type E3 ubiquitin transferase RNF168; RNF168; anti-RNF168 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
571
Applicable Applications for anti-RNF168 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human RNF168 recombinant protein (Position: K5-K571).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of RNF168 using anti-RNF168 antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of RNF168 using anti-RNF168 antibody.)
IHC (Immunohistochemisry)
IHC (Immunohistochemisry)
IF (Immunofluorescence)
IF (Immunofluorescence)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-RNF168 antibody
Description: Rabbit IgG polyclonal antibody for RNF168 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.
Background: Ring finger protein 168 is a protein that in humans is encoded by the RNF168 gene. It is mapped to 3q29. This gene encodes an E3 ubiquitin ligase protein that contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The protein is involved in DNA double-strand break (DSB) repair. Mutations in this gene result in Riddle syndrome.
Background: Ring finger protein 168 is a protein that in humans is encoded by the RNF168 gene. It is mapped to 3q29. This gene encodes an E3 ubiquitin ligase protein that contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The protein is involved in DNA double-strand break (DSB) repair. Mutations in this gene result in Riddle syndrome.
References
1. Devgan, S. S., Sanal, O., Doil, C., Nakamura, K., Nahas, S. A., Pettijohn, K., Bartek, J., Lukas, C., Lukas, J., Gatti, R. A. Homozygous deficiency of ubiquitin-ligase ring-finger protein RNF168 mimics the radiosensitivity syndrome of ataxia-telangiectasia. Cell Death Diff. 18: 1500-1506, 2011.
2. Doil, C., Mailand, N., Bekker-Jensen, S., Menard, P., Larsen, D. H., Pepperkok, R., Ellenberg, J., Panier, S., Durocher, D., Bartek, J., Lukas, J., Lukas, C. RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Cell 136: 435-446, 2009.
3. Nakada, S., Tai, I., Panier, S., Al-Hakim, A., Iemura, S., Juang, Y.-C., O'Donnell, L., Kumakubo, A., Munro, M., Sicheri, F., Gingras, A.-C., Natsume, T., Suda, T., Durocher, D. Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. Nature 466: 941-946, 2010.
2. Doil, C., Mailand, N., Bekker-Jensen, S., Menard, P., Larsen, D. H., Pepperkok, R., Ellenberg, J., Panier, S., Durocher, D., Bartek, J., Lukas, J., Lukas, C. RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Cell 136: 435-446, 2009.
3. Nakada, S., Tai, I., Panier, S., Al-Hakim, A., Iemura, S., Juang, Y.-C., O'Donnell, L., Kumakubo, A., Munro, M., Sicheri, F., Gingras, A.-C., Natsume, T., Suda, T., Durocher, D. Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. Nature 466: 941-946, 2010.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF168
NCBI Official Synonym Full Names
ring finger protein 168
NCBI Official Symbol
RNF168
NCBI Official Synonym Symbols
RIDL; hRNF168
NCBI Protein Information
E3 ubiquitin-protein ligase RNF168
UniProt Protein Name
E3 ubiquitin-protein ligase RNF168
UniProt Gene Name
RNF168
UniProt Synonym Gene Names
hRNF168
UniProt Entry Name
RN168_HUMAN
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Product Notes
The RNF168 rnf168 (Catalog #AAA125419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RNF168 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF168 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RNF168 rnf168 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF168, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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