FCM/FACS (Flow Cytometry)
(RNF8 Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human RNF8 Polyclonal Antibody | anti-RNF8 antibody
RNF8 Antibody
Gene Names
RNF8; hRNF8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF8, Antibody; RNF8 Antibody; E3 ubiquitin-protein ligase RNF8; hRNF8; 632-; RING finger protein 8; RNF8; KIAA0646; anti-RNF8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RNF8 antibody is generated from rabbits immunized with recombinant protein of human RNF8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.44 (varies by lot)
Sequence Length
485
Applicable Applications for anti-RNF8 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RNF8 Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RNF8 Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of RNF8 Antibody in Jurkat cell line lysates (35ug/lane). RNF8 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of RNF8 Antibody in Jurkat cell line lysates (35ug/lane). RNF8 (arrow) was detected using the purified Pab.)
Related Product Information for anti-RNF8 antibody
RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.
Product Categories/Family for anti-RNF8 antibody
References
Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010)
Ramachandran, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):809-814(2010)
Marteijn, J.A., et al. J. Cell Biol. 186(6):835-847(2009)
Gong, Z., et al. J. Biol. Chem. 284(11):7284-7293(2009)
Zhang, S., et al. Cell Cycle 7(21):3399-3404(2008)
Ramachandran, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):809-814(2010)
Marteijn, J.A., et al. J. Cell Biol. 186(6):835-847(2009)
Gong, Z., et al. J. Biol. Chem. 284(11):7284-7293(2009)
Zhang, S., et al. Cell Cycle 7(21):3399-3404(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55518
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF8 isoform 1
NCBI Official Synonym Full Names
ring finger protein 8, E3 ubiquitin protein ligase
NCBI Official Symbol
RNF8
NCBI Official Synonym Symbols
hRNF8
NCBI Protein Information
E3 ubiquitin-protein ligase RNF8
UniProt Protein Name
E3 ubiquitin-protein ligase RNF8
UniProt Gene Name
RNF8
UniProt Synonym Gene Names
KIAA0646; hRNF8
UniProt Entry Name
RNF8_HUMAN
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Product Notes
The RNF8 rnf8 (Catalog #AAA289239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF8 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RNF8 rnf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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