IF (Immunofluorescence)
(Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 ug/mL.)
Rabbit RNF8 Polyclonal Antibody | anti-RNF8 antibody
RNF8 Antibody
Gene Names
RNF8; hRNF8
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
RNF8, Antibody; RNF8 Antibody; Ring finger protein 8; anti-RNF8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
485
Applicable Applications for anti-RNF8 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a 14 amino acid peptide near the carboxy terminus of human RNF8.
Target Name
RNF8
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 ug/mL.)
IF (Immunofluorescence)
(Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 ug/mL.)
WB (Western Blot)
(Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) and (B) 2 ug/mL.)
WB (Western Blot)
(Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) and (B) 2 ug/mL.)
Related Product Information for anti-RNF8 antibody
RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.
Product Categories/Family for anti-RNF8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,829 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF8 isoform 1
NCBI Official Synonym Full Names
ring finger protein 8, E3 ubiquitin protein ligase
NCBI Official Symbol
RNF8
NCBI Official Synonym Symbols
hRNF8
NCBI Protein Information
E3 ubiquitin-protein ligase RNF8
UniProt Protein Name
E3 ubiquitin-protein ligase RNF8
UniProt Gene Name
RNF8
UniProt Synonym Gene Names
KIAA0646; hRNF8
UniProt Entry Name
RNF8_HUMAN
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Product Notes
The RNF8 rnf8 (Catalog #AAA305106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF8 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RNF8 rnf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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