FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-ROCK2 antibody (AAA127029).Overlay histogram showing A431 cells stained with AAA127029 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROCK2 Antibody (AAA127029, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit ROCK2 Polyclonal Antibody | anti-ROCK2 antibody
Anti-ROCK2 Antibody Picoband
Gene Names
ROCK2; ROCK-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
ROCK2, Antibody; Anti-ROCK2 Antibody Picoband; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; complement C3b/C4b receptor 1 (Knops blood group); Complement receptor type 1; C3b/C4b receptor; CD35; CR1; C3BR; anti-ROCK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-ROCK2 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Immunogen
E coli-derived human ROCK2 recombinant protein (Position: R908-K1386).
Subcellular Localization
Cytoplasm
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728). (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-ROCK2 antibody (AAA127029).Overlay histogram showing A431 cells stained with AAA127029 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROCK2 Antibody (AAA127029, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-ROCK2 antibody (AAA127029).Overlay histogram showing A431 cells stained with AAA127029 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROCK2 Antibody (AAA127029, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of ROCK2 using anti-ROCK2 antibody (AAA127029).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (#AAA127029) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 180 kDa. The expected band size for ROCK2 is at 161 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of ROCK2 using anti-ROCK2 antibody (AAA127029).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (#AAA127029) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 180 kDa. The expected band size for ROCK2 is at 161 kDa.)
Related Product Information for anti-ROCK2 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-ROCK2 antibody
References
1. Takahashi, N.; Tuiki, H.; Saya, H.; Kaibuchi, K. : Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 55: 235-237, 1999. 2. Thumkeo, D.; Keel, J.; Ishizaki, T.; Hirose, M.; Nonomura, K.; Oshima, H.; Oshima, M.; Taketo, M. M.; Narumiya, S. : Targeted disruption of the mouse Rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death. Molec. Cell. Biol. 23: 5043-5055, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160,900 Da
NCBI Official Full Name
rho-associated protein kinase 2
NCBI Official Synonym Full Names
Rho-associated, coiled-coil containing protein kinase 2
NCBI Official Symbol
ROCK2
NCBI Official Synonym Symbols
ROCK-II
NCBI Protein Information
rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II
UniProt Protein Name
Rho-associated protein kinase 2
UniProt Gene Name
ROCK2
UniProt Synonym Gene Names
KIAA0619; ROCK-II
UniProt Entry Name
ROCK2_HUMAN
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Product Notes
The ROCK2 rock2 (Catalog #AAA127029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ROCK2 Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ROCK2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the ROCK2 rock2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROCK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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