IF (Immunofluorescence)
(AAA327356 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse ROR2 Polyclonal Antibody | anti-ROR2 antibody
ROR2 Antibody
Gene Names
ROR2; BDB; BDB1; NTRKR2
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ROR2, Antibody; ROR2 Antibody; BDB; BDB1; Brachydactyly type B; EC 2.7.10.1; MGC163394; Neurotrophic tyrosine kinase; Neurotrophic tyrosine kinase, receptor related 2; NTRKR2; Receptor tyrosine kinase-like orphan receptor 2; receptor-related 2; ROR2; ROR2_HUMAN; Tyrosine protein kinase transmembrane receptor ROR2; Tyrosine-protein kinase transmembrane receptor Ror2; anti-ROR2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ROR2 antibody detects endogenous levels of total ROR2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
432
Applicable Applications for anti-ROR2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ROR2
Subcellular Location
Plasma Membrane
Tissue Specificity
Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA327356 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA327356 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of ROR2 using HeLa whole lysates.)
WB (Western Blot)
(Western blot analysis of ROR2 using HeLa whole lysates.)
Related Product Information for anti-ROR2 antibody
Function: Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development (By similarity). Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation (PubMed:17717073). In contrast, has also been shown to have very little tyrosine kinase activity in vitro. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443).
Subunit Structure: Homodimer; promotes osteogenesis. Binds YWHAB (PubMed:17717073). Interacts with WTIP (By similarity).
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Subunit Structure: Homodimer; promotes osteogenesis. Binds YWHAB (PubMed:17717073). Interacts with WTIP (By similarity).
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 105 kDa
Predicted: 105 kDa
Predicted: 105 kDa
NCBI Official Full Name
tyrosine-protein kinase transmembrane receptor ROR2 isoform 2
NCBI Official Synonym Full Names
receptor tyrosine kinase like orphan receptor 2
NCBI Official Symbol
ROR2
NCBI Official Synonym Symbols
BDB; BDB1; NTRKR2
NCBI Protein Information
tyrosine-protein kinase transmembrane receptor ROR2
UniProt Protein Name
Tyrosine-protein kinase transmembrane receptor ROR2
UniProt Gene Name
ROR2
UniProt Synonym Gene Names
NTRKR2
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Product Notes
The ROR2 ror2 (Catalog #AAA327356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROR2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ROR2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the ROR2 ror2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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