IHC (Immunohiostchemistry)
(Staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit ROS Polyclonal Antibody | anti-ROS antibody
Phospho-ROS (Tyr2114) Antibody
Gene Names
ROS1; ROS; MCF3; c-ros-1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (100%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
ROS, Antibody; Phospho-ROS (Tyr2114) Antibody; c ros 1; c ros; c ros oncogene 1 receptor tyrosine kinase; c Ros receptor tyrosine kinase; c ros1; MCF 3; MCF3; Oncogene ROS; OTTHUMP00000017814; OTTHUMP00000017815; OTTHUMP00000040389; Proto oncogene c ros 1 protein; Proto oncogene c Ros; Proto oncogene tyrosine protein kinase ROS; Proto oncogene tyrosine protein kinase ROS precursor; Proto-oncogene c-Ros-1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c ros oncogene 1; Ros 1; ROS 1C; ROS; ROS proto oncogene 1, receptor tyrosine kinase; ROS_HUMAN; ROS1; ROS1C; Transmembrane tyrosine specific protein kinase; v ros avian UR2 sarcoma virus oncogene homolog 1; v ros UR2 sarcoma virus oncogene homolog 1; anti-ROS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-ROS (Tyr2114) Antibody detects endogenous levels of ROS only when phosphorylated at Tyr2114.
Tissue Specificity: Expressed in brain. Expression is increased in primary gliomas.
Tissue Specificity: Expressed in brain. Expression is increased in primary gliomas.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ROS antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ROS around the phosphorylation site of Tyr2114.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.
Subunit Structure
Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation (By similarity). Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway. Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation.
Similarity
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Subcellular Location
Cell membrane>Single-pass type I membrane protein.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(Staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of ROS (Phospho-Tyr2114) using TNF treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of ROS (Phospho-Tyr2114) using TNF treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-ROS antibody
Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
263,915 Da
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase ROS
NCBI Official Synonym Full Names
ROS proto-oncogene 1, receptor tyrosine kinase
NCBI Official Symbol
ROS1
NCBI Official Synonym Symbols
ROS; MCF3; c-ros-1
NCBI Protein Information
proto-oncogene tyrosine-protein kinase ROS
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase ROS
UniProt Gene Name
ROS1
UniProt Synonym Gene Names
MCF3; ROS
UniProt Entry Name
ROS1_HUMAN
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Product Notes
The ROS ros1 (Catalog #AAA329919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ROS (Tyr2114) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's ROS can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ROS ros1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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