Standard Curve (Sample)
Non-Wash Mitochondrial ROS Activity Assay Kit
Elite Non-Wash Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence), superoxide and hydroxyl radical preferred
Synonyms
Non-Wash Mitochondrial ROS Activity; N/A; Elite Non-Wash Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence), superoxide and hydroxyl radical preferred; Non-Wash Mitochondrial ROS Activity assay kit
Detection
Microplate microplate reader (Ex/Em = 650/675 nm); Fluorescence microscope; Flow cytometer
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Non-Wash Mitochondrial ROS Activity assay kit
Principle of the Assay: Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling and homeostasis. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. This Elite™ Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify mitochondrial ROS in live cells. Elite™ ROS Deep Red uses a membrane-permeable non-fluorescent ROS sensor, which quickly penetrates mitochondrial membrane and generates very strong deep-red fluorescence signal when it reacts with ROS. The kit is an optimized "mix and read" assay format that is compatible with HTS liquid handling instruments. This Kit provides a sensitive, one-step fluorimetric assay to detect mitochondrial ROS (especially superoxide and hydroxyl radicals) in live cells with 1 hour incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and be easily adapted to automation without a wash step. The intensity of the signal can be easily measured using a fluorescence microplate reader at Ex/Em = 650/675 nm, or a fluorescent microscope with TRITC filter or a flow-cytometry in FL2 channel. This kit can be used to either quantify the ROS activities in cells or screen the ROS inhibitors.
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Product Notes
The Non-Wash Mitochondrial ROS Activity (Catalog #AAA62160) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Non-Wash Mitochondrial ROS Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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