IHC (Immunohistochemisry)
(RPL27 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit RPL27 Polyclonal Antibody | anti-RPL27 antibody
RPL27 Antibody (C-term)
Gene Names
RPL27; L27
Reactivity
Human, Mouse (Predicted Reactivity: Bovine, Chicken, Zebrafish, Monkey, Pig, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPL27, Antibody; RPL27 Antibody (C-term); 60S ribosomal protein L27; RPL27; anti-RPL27 antibody
Host
Rabbit
Reactivity
Human, Mouse (Predicted Reactivity: Bovine, Chicken, Zebrafish, Monkey, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RPL27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the C-terminal region of human RPL27.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
91-120
Sequence Length
136
Applicable Applications for anti-RPL27 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25957688, PubMed:25901680). Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs (PubMed:25424902).
Cellular Location
Cytoplasm, cytosol. Cytoplasm. Rough endoplasmic reticulum {ECO:0000250|UniProtKB:A1XQU5} Note=Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). {ECO:0000250|UniProtKB:A1XQU5, ECO:0000269|PubMed:25957688}
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(RPL27 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(RPL27 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(RPL27 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the RPL27 antibody detected the RPL27 protein (arrow).)
WB (Western Blot)
(RPL27 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the RPL27 antibody detected the RPL27 protein (arrow).)
WB (Western Blot)
(Anti-RPL27 Antibody (C-term) at 1:4000 dilution + RPMI 8226 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-RPL27 Antibody (C-term) at 1:4000 dilution + RPMI 8226 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RPL27 antibody
Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L27E family of ribosomal proteins. It is located in the
cytoplasm. As is typical for genes encoding ribosomal proteins,
there are multiple processed pseudogenes of this gene dispersed
through the genome.
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L27E family of ribosomal proteins. It is located in the
cytoplasm. As is typical for genes encoding ribosomal proteins,
there are multiple processed pseudogenes of this gene dispersed
through the genome.
References
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
Yoshihama, M., et al. Genome Res. 12(3):379-390(2002)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
Yoshihama, M., et al. Genome Res. 12(3):379-390(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15798
NCBI Official Full Name
60S ribosomal protein L27
NCBI Official Synonym Full Names
ribosomal protein L27
NCBI Official Symbol
RPL27
NCBI Official Synonym Symbols
L27
NCBI Protein Information
60S ribosomal protein L27
UniProt Protein Name
60S ribosomal protein L27
UniProt Gene Name
RPL27
UniProt Entry Name
RL27_HUMAN
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Product Notes
The RPL27 rpl27 (Catalog #AAA287981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 91-120. The RPL27 Antibody (C-term) reacts with Human, Mouse (Predicted Reactivity: Bovine, Chicken, Zebrafish, Monkey, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RPL27 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RPL27 rpl27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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