WB (Western Blot)
(Detection of human RPS3 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPS3 antibody AAA212309 (lot AAA212309-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human RPS3 Polyclonal Antibody | anti-RPS3 antibody
Rabbit anti-RPS3 Antibody, Affinity Purified
Gene Names
RPS3; S3
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RPS3, Antibody; Rabbit anti-RPS3 Antibody, Affinity Purified; 40S ribosomal protein S3; IMR-90 ribosomal protein S3; S3; small ribosomal subunit protein uS3; ribosomal protein S3; anti-RPS3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RPS3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Bovine, Dog, Pig, Orangutan
Immunogen
Between 193 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RPS3 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPS3 antibody AAA212309 (lot AAA212309-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human RPS3 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPS3 antibody AAA212309 (lot AAA212309-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-RPS3 antibody AAA212309 (lot AAA212309-1) used for IP at 6 ug/mg lysate. RPS3 was also immunoprecipitated by rabbit anti-RPS3 antibody For blotting immunoprecipitated RPS3, AAA212309 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-RPS3 antibody AAA212309 (lot AAA212309-1) used for IP at 6 ug/mg lysate. RPS3 was also immunoprecipitated by rabbit anti-RPS3 antibody For blotting immunoprecipitated RPS3, AAA212309 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human RPS3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPS3 (Cat. No. Lot1, left panel and Cat. No. AAA212309 Lot1, right panel) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RPS3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPS3 (Cat. No. Lot1, left panel and Cat. No. AAA212309 Lot1, right panel) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-RPS3 antibody
RPS3 (40S ribosomal protein S3) has been identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. It has also been identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P23396].
Product Categories/Family for anti-RPS3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,688 Da
NCBI Official Full Name
40S ribosomal protein S3 isoform 1
NCBI Official Synonym Full Names
ribosomal protein S3
NCBI Official Symbol
RPS3
NCBI Official Synonym Symbols
S3
NCBI Protein Information
40S ribosomal protein S3; IMR-90 ribosomal protein S3
UniProt Protein Name
40S ribosomal protein S3
UniProt Gene Name
RPS3
UniProt Entry Name
RS3_HUMAN
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Product Notes
The RPS3 rps3 (Catalog #AAA212309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPS3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPS3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPS3 rps3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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