WB (Western Blot)
(Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody AAA211020 (lot AAA211020-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human, Mouse RPS6 Polyclonal Antibody | anti-RPS6 antibody
Rabbit anti-RPS6 Antibody, Affinity Purified
Gene Names
RPS6; S6
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RPS6, Antibody; Rabbit anti-RPS6 Antibody, Affinity Purified; S6; phosphoprotein NP33; 40S ribosomal protein S6; small ribosomal subunit protein eS6; ribosomal protein S6; anti-RPS6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-RPS6 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, X. laevis, Chicken, Bovine
Immunogen
Between 199 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody AAA211020 (lot AAA211020-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody AAA211020 (lot AAA211020-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody AAA211020 (lot AAA211020-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot AAA211020-1). For blotting immunoprecipitated RPS6, AAA211020 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody AAA211020 (lot AAA211020-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot AAA211020-1). For blotting immunoprecipitated RPS6, AAA211020 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human RPS6 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (Cat. No. AAA211020 lot 2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RPS6 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (Cat. No. AAA211020 lot 2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-RPS6 antibody
Ribosomal protein S6 (RPS6) is a component of the 40S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPS6 is phosphorylated by many cellular kinases including ribosomal protein S6 kinase that is regulated via the mammalian target of rapamycin (mTOR) pathway. RPS6 has been demonstrated to be important in regulating cell size, glucose homeostasis, and translational control of mRNA.
Product Categories/Family for anti-RPS6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,681 Da
NCBI Official Full Name
40S ribosomal protein S6
NCBI Official Synonym Full Names
ribosomal protein S6
NCBI Official Symbol
RPS6
NCBI Official Synonym Symbols
S6
NCBI Protein Information
40S ribosomal protein S6; phosphoprotein NP33
UniProt Protein Name
40S ribosomal protein S6
UniProt Gene Name
RPS6
UniProt Entry Name
RS6_HUMAN
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Product Notes
The RPS6 rps6 (Catalog #AAA211020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPS6 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPS6 rps6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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