Rabbit RPS6 Polyclonal Antibody | anti-RPS6 antibody

Anti-RPS6 Antibody, Rabbit Polyclonal

Cat. Num. AAA254305

• Gene Names: RPS6; S6
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: RPS6, Antibody; Anti-RPS6 Antibody, Rabbit Polyclonal; RPS6 Antibody, Rabbit PAb, Antigen Affinity Purified; ribosomal protein S6; Anti-S6 Antibody; anti-RPS6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RPS6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RPS6 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human RPS6
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RPS6, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(Immunochemical staining RPS6 in rat pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining RPS6 in mouse pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemisry)

(Immunochemical staining RPS6 in human pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining RPS6 in human colon carcinoma with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-RPS6 rabbit polyclonal antibody at 1:2000 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:32 kDa)

Related Product Information for anti-RPS6 antibody

Ribosomal protein S6 (rpS6), a component of the 40S ribosomal subunit, is involved in multiple cellular bioactivities. RPS6 is a downstream substrate of PI3K/Akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double-strand breaks. The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES.

Product Categories/Family for anti-RPS6 antibody

AMPK Signaling Pathway

References

Wu S, et al. (2019) Mtorc1/rps6 and spermatogenic function in the testis-insights from the adjudin model. Reprod Toxicol 89 54-66.Son O, et al. (2015) Identification of nucleosome assembly protein 1 (nap1) as an interacting partner of plant ribosomal protein s6 (rps6) and a positive regulator of rdna transcription. Biochem Biophys Res Commun 465 (2): 200-205.Grundy M, et al. (2018) Early changes in rps6 phosphorylation and bh3 profiling predict response to chemotherapy in aml cells. PLoS One 13 (5): e0196805.

NCBI and Uniprot Product Information

• NCBI GI #: 17158044
• NCBI GeneID: 6194
• NCBI Accession #: NP_001001.2
• NCBI GenBank Nucleotide #: NM_001010.2
• UniProt Accession #: P62753; P08227; P10660; Q4VBY7; Q8N6Z7
• Molecular Weight: 28,681 Da
• NCBI Official Full Name: 40S ribosomal protein S6
• NCBI Official Synonym Full Names: ribosomal protein S6
• NCBI Official Symbol: RPS6
• NCBI Official Synonym Symbols: S6
• NCBI Protein Information: 40S ribosomal protein S6; phosphoprotein NP33
• UniProt Protein Name: 40S ribosomal protein S6
• UniProt Gene Name: RPS6
• UniProt Entry Name: RS6_HUMAN