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IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using RUNX1 antibody. Blue: DAPI for nuclear staining.)
Rabbit RUNX1 Polyclonal Antibody | anti-RUNX1 antibody
RUNX1 Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
RUNX1; Polyclonal Antibody; RUNX1 Antibody; AML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; anti-RUNX1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total RUNX1 protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
453
Applicable Applications for anti-RUNX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunoprecipitation: 1:20 - 1:100
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunoprecipitation: 1:20 - 1:100
Immunogen Type
Peptide
Immunogen Description
A synthetic peptide of human RUNX1.
Target Name
RUNX1
Preparation and Storage
Store at -20 degree C
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using RUNX1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using RUNX1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat kidney using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat kidney using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain using RUNX1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain using RUNX1 antibody at dilution of 1:100 (400x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RUNX1antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RUNX1antibody.)
Related Product Information for anti-RUNX1 antibody
AML1 (also known as Runx1, CBFA2, and PEBP2aB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFb and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
Product Categories/Family for anti-RUNX1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,733 Da
NCBI Official Full Name
runt-related transcription factor 1 isoform AML1b
NCBI Official Synonym Full Names
runt-related transcription factor 1
NCBI Official Symbol
RUNX1
NCBI Official Synonym Symbols
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
NCBI Protein Information
runt-related transcription factor 1
UniProt Protein Name
Runt-related transcription factor 1
UniProt Gene Name
RUNX1
UniProt Synonym Gene Names
AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
UniProt Entry Name
RUNX1_HUMAN
Similar Products
Product Notes
The RUNX1 runx1 (Catalog #AAA29718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUNX1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RUNX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:100. Researchers should empirically determine the suitability of the RUNX1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUNX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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