WB (Western Blot)
(Anti-Runx1 Antibody (C-term)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human Runx1 Polyclonal Antibody | anti-Runx1 antibody
Mouse Runx1 Antibody (C-term)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Runx1, Antibody; Mouse Runx1 Antibody (C-term); Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; Runx1; Aml1; Cbfa2; Pebp2ab; anti-Runx1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Runx1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 305-339 amino acids from the C-terminal region of Mouse Runx1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
451
Applicable Applications for anti-Runx1 antibody
ELISA, WB (Western Blot)
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-Runx1 Antibody (C-term)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Runx1 Antibody (C-term)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Runx1 Antibody (C-term)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Runx1 Antibody (C-term)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Runx1 antibody
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B- dependent transcriptional activation (By similarity).
Product Categories/Family for anti-Runx1 antibody
References
Bae S.-C.,et al.Oncogene 8:809-814(1993).
Bae S.-C.,et al.Mol. Cell. Biol. 14:3242-3252(1994).
Calabi F.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Tsuji K.,et al.Biochem. Biophys. Res. Commun. 274:171-176(2000).
Fujita Y.,et al.Biochem. Biophys. Res. Commun. 281:1248-1255(2001).
Bae S.-C.,et al.Mol. Cell. Biol. 14:3242-3252(1994).
Calabi F.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Tsuji K.,et al.Biochem. Biophys. Res. Commun. 274:171-176(2000).
Fujita Y.,et al.Biochem. Biophys. Res. Commun. 281:1248-1255(2001).
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
48610
NCBI Official Full Name
Runt-related transcription factor 1
UniProt Protein Name
Runt-related transcription factor 1
UniProt Gene Name
Runx1
UniProt Synonym Gene Names
Aml1; Cbfa2; Pebp2ab; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
UniProt Entry Name
RUNX1_MOUSE
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Product Notes
The Runx1 runx1 (Catalog #AAA285818) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Runx1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Runx1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Runx1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Runx1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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