FCM/FACS (Flow Cytometry)
(Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (bs-2696R) Dilution: 2 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Rabbit anti-Rat S100-A8/MRP8 Polyclonal Antibody | anti-S100-A8/MRP8 antibody
S100-A8/MRP8 Polyclonal Antibody
Gene Names
S100a8; p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
Reactivity
Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
S100-A8/MRP8, Antibody; S100-A8/MRP8 Polyclonal Antibody; Protein S100-A8; Calgranulin-A; Chemotactic cytokine CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; S100 calcium-binding protein A8; S100a8; Caga; Mrp8; anti-S100-A8/MRP8 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-S100-A8/MRP8 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Notes
ECO:0000312|MGI: MGI:88244
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (bs-2696R) Dilution: 2 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (bs-2696R) Dilution: 2 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control:K562. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (bs-2696R) Dilution: 2 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control:K562. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (bs-2696R) Dilution: 2 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein S100-A8
NCBI Official Synonym Full Names
S100 calcium binding protein A8 (calgranulin A)
NCBI Official Symbol
S100a8
NCBI Official Synonym Symbols
p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
NCBI Protein Information
protein S100-A8; MRP-8; S100 calcium-binding protein A8; calgranulin-A; chemotactic S100 protein; chemotactic cytokine CP-10; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; pro-inflammatory S100 cytokine
UniProt Protein Name
Protein S100-A8
UniProt Gene Name
S100a8
UniProt Synonym Gene Names
Caga; Mrp8; MRP-8; p8
UniProt Entry Name
S10A8_MOUSE
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Product Notes
The S100-A8/MRP8 s100a8 (Catalog #AAA291954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S100-A8/MRP8 Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100-A8/MRP8 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the S100-A8/MRP8 s100a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100-A8/MRP8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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