Goat S100A9 / MRP14 Polyclonal Antibody | anti-S100A9 antibody

Goat Polyclonal to Human S100A9 / MRP14

Cat. Num. AAA50999

• Gene Names: S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Synonyms: S100A9 / MRP14, Antibody; Goat Polyclonal to Human S100A9 / MRP14; Anti-S100A9 / MRP14 Antibody (Internal) IHC-plus; S100A9; 60B8AG; Calgranulin B; CFAG; CAGB; Calgranulin-B; Calprotectin L1H subunit; CGLB; L1Ag; MRP14; NIF; p14; Protein S100-A9; LIAG; MAC387; MRP-14; Human S100A9; anti-S100A9 antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: Human S100A9.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 114

• Applicable Applications for anti-S100A9 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Epitope: aa67-79
• Immunogen: S100A9 / MRP14 was raised against peptide with sequence C-DTNADKQLSFEEF, from the internal region of the protein sequence according to NP_002956.1.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

WB (Western Blot)

(Antibody (0.5 ug/ml) staining of Human Peripheral Mononucleocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohiostchemistry)

(Anti-S100A9 / MRP14 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-S100A9 / MRP14 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Related Product Information for anti-S100A9 antibody

Immunohistochemistry: MBS242631 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242631 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16;280(50):41521-9.; PMID: 16216873). Recommended concentration: 0.3-1 ug/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 4506773
• NCBI GeneID: 6280
• NCBI Accession #: NP_002956.1
• NCBI GenBank Nucleotide #: NM_002965.3
• UniProt Accession #: P06702; Q6FGA1; Q9NYM0; Q9UCJ1; D3DV36
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9
• NCBI Official Symbol: S100A9
• NCBI Official Synonym Symbols: MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• NCBI Protein Information: protein S100-A9; MRP-14; calgranulin B; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B)
• UniProt Protein Name: Protein S100-A9
• UniProt Gene Name: S100A9
• UniProt Synonym Gene Names: CAGB; CFAG; MRP14; MRP-14; p14
• UniProt Entry Name: S10A9_HUMAN