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IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with SCAP (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA289267 SCAP (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
Rabbit SCAP Polyclonal Antibody | anti-SCAP antibody
SCAP Antibody (Center)
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SCAP, Antibody; SCAP Antibody (Center); Sterol regulatory element-binding protein cleavage-activating protein; SCAP; SREBP cleavage-activating protein; KIAA0199; anti-SCAP antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SCAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-632 amino acids from the Central region of human SCAP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
604-632
Sequence Length
1279
Applicable Applications for anti-SCAP antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with SCAP (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA289267 SCAP (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with SCAP (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA289267 SCAP (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using SCAP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using SCAP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human colon carcinoma with SCAP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human colon carcinoma with SCAP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of SCAP Antibody (Center) in K562 cell line lysates (35ug/lane). SCAP (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SCAP Antibody (Center) in K562 cell line lysates (35ug/lane). SCAP (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of lysate from PC-3 cell line, using SCAP Antibody (Center). AAA289267 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from PC-3 cell line, using SCAP Antibody (Center). AAA289267 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-SCAP antibody
SCAP is a protein with a sterol sensing domain(SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.
Product Categories/Family for anti-SCAP antibody
References
Lu,Y., et.al., J. Lipid Res. 49 (12), 2582-2589 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
139729
NCBI Official Full Name
sterol regulatory element-binding protein cleavage-activating protein
NCBI Official Synonym Full Names
SREBF chaperone
NCBI Official Symbol
SCAP
NCBI Protein Information
sterol regulatory element-binding protein cleavage-activating protein
UniProt Protein Name
Sterol regulatory element-binding protein cleavage-activating protein
UniProt Gene Name
SCAP
UniProt Synonym Gene Names
KIAA0199; SCAP; SREBP cleavage-activating protein
UniProt Entry Name
SCAP_HUMAN
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Product Notes
The SCAP scap (Catalog #AAA289267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 604-632. The SCAP Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SCAP can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SCAP scap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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