WB (Western Blot)
(Anti-SDHB antibody, AAA45161, Western blottingAll lanes: Anti SDHB (AAA45161) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: Jurkat Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 32KD)
Rabbit SDHB Polyclonal Antibody | anti-SDHB antibody
Anti-SDHB antibody
Gene Names
SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
SDHB, Antibody; Anti-SDHB antibody; Succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex, subunit B, iron sulfur(Ip); CWS2 antibody; DHSB_HUMAN antibody; FLJ92337 antibody; IP antibody; Iron sulfur subunit antibody; Iron sulfur subunit of complex II antibody; Iron-sulfur subunit of complex II antibody; mitochondrial antibody; OTTHUMP00000044624 antibody; PGL 4 antibody; PGL4 antibody; SDH 1 antibody; SDH antibody; SDH1 antibody; SDH2 antibody; SDHB antibody; SDHIP antibody; Succinate dehydrogenase[ubiquinone] iron sulfur protein mitochondrial antibody; Succinate dehydrogenase[ubiquinone] iron-sulfur subunit antibody; succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial antibody; Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody; Succinate dehydrogenase complex subunit B iron sulfur antibody; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody; succinate dehydrogenase complex, subunit B, iron sulfur(Ip) antibody; Succinate dehydrogenase iron sulfur protein antibody; anti-SDHB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
280
Applicable Applications for anti-SDHB antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SDHB (42-59 FAIYRWDPDKAGDKPHMQ), different from the related rat sequence by one amino acid and mouse sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-SDHB antibody, AAA45161, Western blottingAll lanes: Anti SDHB (AAA45161) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: Jurkat Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 32KD)
WB (Western Blot)
(Anti-SDHB antibody, AAA45161, Western blottingAll lanes: Anti SDHB (AAA45161) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: Jurkat Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 32KD)
IHC (Immunohistochemistry)
(Anti-SDHB antibody, AAA45161, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-SDHB antibody, AAA45161, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
Related Product Information for anti-SDHB antibody
Description: Rabbit IgG polyclonal antibody for Succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial(SDHB) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: SDHB(Succinate Dehydrogenase Complex, Subunit B, iron sulfur protein), also known as iron-sulfur subunit of complex II(Ip) or SDH2, HOMOLOG OF, is a protein that in humans is encoded by the SDHB gene. SDHB is one of four protein subunits forming succinate dehydrogenase, the other three being SDHA, SDHC and SDHD. The SDHB subunit is connected to the SDHA subunit on the hydrophilic, catalytic end of the SDH complex. The SDHB gene is mapped on 1p36.13. The entire SDHB transcript is encoded by 8 exons within approximately 40 kb by Au et al(1995). Pollard et al.(2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase and succinate dehydrogenases like SDHB act as tumor suppressors, and germline mutations in these genes predispose individuals to leiomyomas and renal cancer and to paragangliomas, respectively. In affected members of families with paragangliomas-4, mutations were identified in the SDHB gene.
Background: SDHB(Succinate Dehydrogenase Complex, Subunit B, iron sulfur protein), also known as iron-sulfur subunit of complex II(Ip) or SDH2, HOMOLOG OF, is a protein that in humans is encoded by the SDHB gene. SDHB is one of four protein subunits forming succinate dehydrogenase, the other three being SDHA, SDHC and SDHD. The SDHB subunit is connected to the SDHA subunit on the hydrophilic, catalytic end of the SDH complex. The SDHB gene is mapped on 1p36.13. The entire SDHB transcript is encoded by 8 exons within approximately 40 kb by Au et al(1995). Pollard et al.(2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase and succinate dehydrogenases like SDHB act as tumor suppressors, and germline mutations in these genes predispose individuals to leiomyomas and renal cancer and to paragangliomas, respectively. In affected members of families with paragangliomas-4, mutations were identified in the SDHB gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,630 Da
NCBI Official Full Name
Succinate dehydrogenase
NCBI Official Synonym Full Names
succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
NCBI Official Symbol
SDHB
NCBI Official Synonym Symbols
IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
NCBI Protein Information
succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; iron-sulfur subunit of complex II
UniProt Protein Name
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Gene Name
SDHB
UniProt Synonym Gene Names
SDH; SDH1; Ip
UniProt Entry Name
SDHB_HUMAN
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Product Notes
The SDHB sdhb (Catalog #AAA45161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SDHB antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SDHB can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SDHB sdhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDHB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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