WB (Western Blot)
(Detection of human SERCA2 by western blot. Samples: Whole cell lysate (50 ug) from A-549, HeLa, HEK293T, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SERCA2 antibody (AAA210933 lot 5) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human SERCA2 Polyclonal Antibody | anti-SERCA2 antibody
Rabbit anti-SERCA2 Antibody, Affinity Purified
Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2; FLJ20293; FLJ38063; MGC45367; DKFZp686P0211
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SERCA2, Antibody; Rabbit anti-SERCA2 Antibody, Affinity Purified; cardiac Ca2+ ATPase; DAR; SR Ca(2+)-ATPase 2; SERCA2; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATP2B; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; calcium pump 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase Ca++ transporting cardiac muscle slow twitch 2; DD; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2; anti-SERCA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SERCA2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Chicken, Dog, Rabbit, Pig, Cat
Immunogen
Between 475 and 525
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SERCA2 by western blot. Samples: Whole cell lysate (50 ug) from A-549, HeLa, HEK293T, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SERCA2 antibody (AAA210933 lot 5) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human SERCA2 by western blot. Samples: Whole cell lysate (50 ug) from A-549, HeLa, HEK293T, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SERCA2 antibody (AAA210933 lot 5) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human SERCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SERCA2 antibody (AAA210933 lot 5) used for IP at 6 ug per reaction. SERCA2 was also immunoprecipitated by a previous lot of this antibody (AAA210933 lot 4). For blotting immunoprecipitated SERCA2, AAA210933 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SERCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SERCA2 antibody (AAA210933 lot 5) used for IP at 6 ug per reaction. SERCA2 was also immunoprecipitated by a previous lot of this antibody (AAA210933 lot 4). For blotting immunoprecipitated SERCA2, AAA210933 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-SERCA2 antibody
Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis.
Product Categories/Family for anti-SERCA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,757 Da
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform b
NCBI Official Synonym Full Names
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
NCBI Official Symbol
ATP2A2
NCBI Official Synonym Symbols
DD; DAR; ATP2B; SERCA2; FLJ20293; FLJ38063; MGC45367; DKFZp686P0211
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 2; calcium pump 2; SR Ca(2+)-ATPase 2; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; calcium-transporting ATPase sarcoplasmic re
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Gene Name
ATP2A2
UniProt Synonym Gene Names
ATP2B; SERCA2
UniProt Entry Name
AT2A2_HUMAN
Similar Products
Product Notes
The SERCA2 atp2a2 (Catalog #AAA210933) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SERCA2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERCA2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SERCA2 atp2a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERCA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.