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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

Rabbit SERPINB1 Polyclonal Antibody | anti-SERPINB1 antibody

SERPINB1 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
SERPINB1; Polyclonal Antibody; SERPINB1 Polyclonal Antibody; SERPINB1;EI;ELANH2;HEL-S-27;HEL57;LEI;M/NEI;MNEI;PI-2;PI2; anti-SERPINB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Sequence Length
379
Applicable Applications for anti-SERPINB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:20-1:100
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human SERPINB1 (NP_109591.1).
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-34304 / sc-34305 / sc-66957
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded rat spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cell using SERPINB1 antibody. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cell using SERPINB1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using SERPINB1 antibody (AAA22061) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SERPINB1 antibody (AAA22061) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using SERPINB1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SERPINB1 antibody.)
Related Product Information for anti-SERPINB1 antibody
The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 45KDa
Calculated MW: 26kDa/42kDa
NCBI Official Full Name
SERPINB1, partial

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Product Notes

The SERPINB1 (Catalog #AAA22061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SERPINB1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:20-1:100. Researchers should empirically determine the suitability of the SERPINB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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