WB (Western Blot)
(Detection of human SF3b145/SAP145 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3b145/SAP145 antibody AAA211550 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
Rabbit anti-Human SF3b145/SAP145 Polyclonal Antibody | anti-SF3b145/SAP145 antibody
Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified
Gene Names
SF3B2; Cus1; SF3b1; SAP145; SF3B145; SF3b150
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SF3b145/SAP145, Antibody; Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified; pre-mRNA splicing factor SF3b 145 kDa subunit; SF3b150; splicing factor 3b, subunit 2, 145kDa; splicing factor 3b, subunit 2, 145kD; splicing factor 3B subunit 2; Spliceosome-associated protein 145; spliceosome associated protein 145; SF3b1; SAP145; Cus1; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SF3B145; SAP 145; Splicing factor 3B subunit 2; splicing factor 3b subunit 2; anti-SF3b145/SAP145 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SF3b145/SAP145 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 845 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SF3b145/SAP145 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3b145/SAP145 antibody AAA211550 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
WB (Western Blot)
(Detection of human SF3b145/SAP145 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3b145/SAP145 antibody AAA211550 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
IHC (Immunohistochemistry)
(Detection of human SF3b145/SAP145 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-SF3b145/SAP145 (Cat. No. AAA211550 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SF3b145/SAP145 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-SF3b145/SAP145 (Cat. No. AAA211550 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-SF3b145/SAP145 antibody
SF3b145/SAP145 is a 145 kDa subunit of the splicing factor 3B (SF3B) protein complex. The SF3B complex is composed of at least 8 subunits (SF3B1 through SF3B8). SF3A along with SF3B and a 12S RNA unit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. In this complex SF3B2 interacts directly with SF3B4 and associates with the pre-mRNA upstream of the branch site at the anchoring site. SF3A and SF3B may play a role in anchoring the U2 snRNP to the pre-mRNA upstream of the intron's branch site.
Product Categories/Family for anti-SF3b145/SAP145 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 100kDa
NCBI Official Full Name
splicing factor 3B subunit 2
NCBI Official Synonym Full Names
splicing factor 3b subunit 2
NCBI Official Symbol
SF3B2
NCBI Official Synonym Symbols
Cus1; SF3b1; SAP145; SF3B145; SF3b150
NCBI Protein Information
splicing factor 3B subunit 2
UniProt Protein Name
High affinity choline transporter 1
UniProt Gene Name
SLC5A7
UniProt Synonym Gene Names
CHT1; CHT
UniProt Entry Name
SC5A7_HUMAN
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Product Notes
The SF3b145/SAP145 slc5a7 (Catalog #AAA211550) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SF3b145/SAP145 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SF3b145/SAP145 slc5a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3b145/SAP145, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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