WB (Western Blot)
(Western blot analysis of extracts from HT-29 cells, using SFRS7 antibody.)
Rabbit anti-Human, Mouse SFRS7 Polyclonal Antibody | anti-SFRS7 antibody
SFRS7 Antibody
Gene Names
SRSF7; 9G8; AAG3; SFRS7
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SFRS7, Antibody; SFRS7 Antibody; 9G8; AAG3; arginine/serine-rich 7; HSSG1; RBM37; Serine/arginine-rich splicing factor 7; Splicing factor 9G8; Splicing factor; Splicing factor, arginine/serine rich 7; SRSF7; SRSF7_HUMAN; ZCCHC20; ZCRB2; anti-SFRS7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SFRS7 antibody detects endogenous levels of total SFRS7
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-SFRS7 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Brain, liver, kidney and lung.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (94%), Xenopus (94%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HT-29 cells, using SFRS7 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HT-29 cells, using SFRS7 antibody.)
IF (Immunofluorescence)
(AAA325191 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA325191 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-SFRS7 antibody
Function: Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.
Subunit Structure: Found in large molecular weight complexes containing CCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interacts with CCNL2 and CPSF6. Interacts with NXF1.
Post-translational Modifications: Extensively phosphorylated on serine residues in the RS domain.
Similarity: Belongs to the splicing factor SR family.
Subunit Structure: Found in large molecular weight complexes containing CCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interacts with CCNL2 and CPSF6. Interacts with NXF1.
Post-translational Modifications: Extensively phosphorylated on serine residues in the RS domain.
Similarity: Belongs to the splicing factor SR family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 35 kD
Predicted: 28 kDa
Predicted: 28 kDa
NCBI Official Full Name
serine/arginine-rich splicing factor 7 isoform 1
NCBI Official Synonym Full Names
serine and arginine rich splicing factor 7
NCBI Official Symbol
SRSF7
NCBI Official Synonym Symbols
9G8; AAG3; SFRS7
NCBI Protein Information
serine/arginine-rich splicing factor 7
UniProt Protein Name
Serine/arginine-rich splicing factor 7
UniProt Gene Name
SRSF7
UniProt Synonym Gene Names
SFRS7
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Product Notes
The SFRS7 srsf7 (Catalog #AAA325191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SFRS7 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SFRS7 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SFRS7 srsf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRS7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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