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IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using SGK1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit SGK1 Polyclonal Antibody | anti-SGK1 antibody
SGK1 Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
SGK1, Antibody; SGK1 Antibody; SGK; SGK1; anti-SGK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total SGK1 protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
526
Applicable Applications for anti-SGK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human SGK1.
Target Name
SGK1
Preparation and Storage
Store at -20°C
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using SGK1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using SGK1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using SGK1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using SGK1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human esophagus using SGK1 at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human esophagus using SGK1 at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human stomach using SGK1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human stomach using SGK1 antibody at dilution of 1:100 (400x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SGK1 at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SGK1 at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SGK1 at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SGK1 at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SGK1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SGK1 antibody.)
Related Product Information for anti-SGK1 antibody
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).
(2) SGK is negatively regulated by ubiquination and proteasome degradation (7). The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).
(2) SGK is negatively regulated by ubiquination and proteasome degradation (7). The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).
Product Categories/Family for anti-SGK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
SDS-PAGE MW: 49KD
NCBI Official Full Name
serine/threonine-protein kinase Sgk1 isoform 2
NCBI Official Synonym Full Names
serum/glucocorticoid regulated kinase 1
NCBI Official Symbol
SGK1
NCBI Official Synonym Symbols
SGK
NCBI Protein Information
serine/threonine-protein kinase Sgk1
UniProt Protein Name
Serine/threonine-protein kinase Sgk1
UniProt Gene Name
SGK1
UniProt Synonym Gene Names
SGK
UniProt Entry Name
SGK1_HUMAN
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Product Notes
The SGK1 sgk1 (Catalog #AAA29714) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SGK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SGK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the SGK1 sgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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