Rabbit anti-Human, Mouse SHANK3 Polyclonal Antibody | anti-SHANK3 antibody

SHANK3 Antibody

Cat. Num. AAA10913

• Gene Names: SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: SHANK3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SHANK3, Antibody; SHANK3 Antibody; SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3; KIAA1650; SH3 and multiple ankyrin repeat domains protein 3; Proline-rich synapse-associated protein 2; Shank3; SH3 and multiple ankyrin repeat domains 3; anti-SHANK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
• Purity/Purification: SHANK3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1,612

• Applicable Applications for anti-SHANK3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)
• Application Notes: SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 ug/mL.; Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL.; For immunofluorescence start at 2.5 ug/mL.; Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples.; All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3.; Immunogen is located withing amino avids 890-940 of SHANK3
• Positive Control: 1) Cat. No. - 3T3 (NIH) Cell Lysate; 2) Human Brain Tissue Slide
• Note: Optimal dilutions for each application to be determined by the researcher.
• Preparation and Storage: SHANK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-SHANK3 antibody

SHANK3 Antibody: SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 122937241
• NCBI GeneID: 85358
• NCBI Accession #: NP_001073889
• NCBI GenBank Nucleotide #: NM_001080420.1
• UniProt Accession #: Q9BYB0; Q8TET3; D7UT47
• NCBI Official Synonym Full Names: SH3 and multiple ankyrin repeat domains 3
• NCBI Official Symbol: SHANK3
• NCBI Official Synonym Symbols: PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 3
• UniProt Protein Name: SH3 and multiple ankyrin repeat domains protein 3
• UniProt Gene Name: SHANK3
• UniProt Synonym Gene Names: KIAA1650; PROSAP2; PSAP2; Shank3; ProSAP2
• UniProt Entry Name: SHAN3_HUMAN