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IF (Immunofluorescence) (Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.)

Rabbit anti-Human, Mouse SHANK3 Polyclonal Antibody | anti-SHANK3 antibody

SHANK3 Antibody

Gene Names
SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SHANK3 Antibody is affinity chromatography purified via peptide column.
Synonyms
SHANK3, Antibody; SHANK3 Antibody; SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3; KIAA1650; SH3 and multiple ankyrin repeat domains protein 3; Proline-rich synapse-associated protein 2; Shank3; SH3 and multiple ankyrin repeat domains 3; anti-SHANK3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
Purity/Purification
SHANK3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
1,612
Applicable Applications for anti-SHANK3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)
Application Notes
SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 ug/mL.
Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL.
For immunofluorescence start at 2.5 ug/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples.
All other applications and species not yet tested.
Conjugate
Unconjugated
Immunogen
SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3.
Immunogen is located withing amino avids 890-940 of SHANK3
Positive Control
1) Cat. No. - 3T3 (NIH) Cell Lysate
2) Human Brain Tissue Slide
Note
Optimal dilutions for each application to be determined by the researcher.
Preparation and Storage
SHANK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

WB (Western Blot) (Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-SHANK3 antibody
SHANK3 Antibody: SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Synonym Full Names
SH3 and multiple ankyrin repeat domains 3
NCBI Official Symbol
SHANK3
NCBI Official Synonym Symbols
PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3
NCBI Protein Information
SH3 and multiple ankyrin repeat domains protein 3
UniProt Protein Name
SH3 and multiple ankyrin repeat domains protein 3
UniProt Gene Name
SHANK3
UniProt Synonym Gene Names
KIAA1650; PROSAP2; PSAP2; Shank3; ProSAP2
UniProt Entry Name
SHAN3_HUMAN

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Product Notes

The SHANK3 shank3 (Catalog #AAA10913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHANK3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHANK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF). SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 2.5 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the SHANK3 shank3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHANK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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