WB (Western Blot)
(Detection of mouse SHANK3 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse SHANK3 Polyclonal Antibody | anti-SHANK3 antibody
Rabbit anti-SHANK3 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SHANK3, Antibody; Rabbit anti-SHANK3 Antibody, Affinity Purified; SH3 and multiple ankyrin repeat domains protein 3; DEL22q13.3; SPANK-2; Shank3; shank postsynaptic density protein; PSAP2; PROSAP2; SCZD15; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; SH3 and multiple ankyrin repeat domains 3; anti-SHANK3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SHANK3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 1475 and 1525
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse SHANK3 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse SHANK3 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human SHANK3 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, SR, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human SHANK3 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, SR, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human SHANK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for IP at 6 ug per reaction. SHANK3 was also immunoprecipitated by a previous lot of this antibody (AAA212533 lot 1). For blotting immunoprecipitated SHANK3, AAA212533 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SHANK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHANK3 antibody (AAA212533 lot 2) used for IP at 6 ug per reaction. SHANK3 was also immunoprecipitated by a previous lot of this antibody (AAA212533 lot 1). For blotting immunoprecipitated SHANK3, AAA212533 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-SHANK3 antibody
SH3 and multiple ankyrin repeat domains 3 (SHANK3) seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK3 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BYB0].
Product Categories/Family for anti-SHANK3 antibody
NCBI and Uniprot Product Information
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Product Notes
The SHANK3 shank3 (Catalog #AAA212533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SHANK3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHANK3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SHANK3 shank3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHANK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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