WB (Western Blot)
(Western blot analysis of lysate from mouse liver tissue lysate, using Shh Antibody (C-term). AAA284928 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit anti-Mouse Shh Polyclonal Antibody | anti-Shh antibody
(Mouse) Shh Antibody (C-term)
Gene Names
Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
Reactivity
Mouse
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Shh, Antibody; (Mouse) Shh Antibody (C-term); Sonic hedgehog protein; SHH; HHG-1; Sonic hedgehog protein N-product; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein C-product; Sonic hedgehog protein 27 kDa product; Shh; Hhg1; anti-Shh antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of mouse Shh.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Length
437
Applicable Applications for anti-Shh antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from mouse liver tissue lysate, using Shh Antibody (C-term). AAA284928 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from mouse liver tissue lysate, using Shh Antibody (C-term). AAA284928 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using (Mouse) Shh Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284928 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using (Mouse) Shh Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284928 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-Shh antibody
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).
Product Categories/Family for anti-Shh antibody
References
Echelard Y.,et al.Cell 75:1417-1430(1993).
McMahon A.P.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Chang D.T.,et al.Development 120:3339-3353(1994).
Carninci P.,et al.Science 309:1559-1563(2005).
Roelink H.,et al.Cell 81:445-455(1995).
McMahon A.P.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Chang D.T.,et al.Development 120:3339-3353(1994).
Carninci P.,et al.Science 309:1559-1563(2005).
Roelink H.,et al.Cell 81:445-455(1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47773
NCBI Official Full Name
sonic hedgehog protein
NCBI Official Synonym Full Names
sonic hedgehog
NCBI Official Symbol
Shh
NCBI Official Synonym Symbols
Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
NCBI Protein Information
sonic hedgehog protein
UniProt Protein Name
Sonic hedgehog protein
UniProt Gene Name
Shh
UniProt Synonym Gene Names
Hhg1; SHH
UniProt Entry Name
SHH_MOUSE
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Product Notes
The Shh shh (Catalog #AAA284928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Shh Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Shh can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Shh shh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Shh, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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