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Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.)
Rabbit SIAH2 Polyclonal Antibody | anti-SIAH2 antibody
SIAH2 antibody - N-terminal region
Gene Names
SIAH2; hSiah2
Reactivity
Tested Species Reactivity: HumanPredicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig
Applications
Western Blot
Purity
Affinity Purified
Synonyms
SIAH2, Antibody; SIAH2 antibody - N-terminal region; anti-SIAH2 antibody
Host
Rabbit
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/ml (varies by lot)
Sequence
Synthetic peptide located within the following region: SRPSSTGPSANKPCSKQPPPQPQHTPSPAAPPAAATISAAGPGSSAVPAA
Sequence Length
324
Applicable Applications for anti-SIAH2 antibody
Western Blot (WB)
Protein Size (# AA)
324 amino acids
Homology
Cow: 93%; Dog: 92%; Human: 100%; Mouse: 80%; Pig: 93%; Rat: 80%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH2
Protein Interactions
EGLN3; XAF1; HIPK2; UBE2D1; UBE2L6; SIAH2; UBE2D3; UBC; PPP1R13B; TP53BP2; NFE2L2; USP19; AR; UBE2I; STAT3; MYD88; EEF1D; CCBE1; SETD2; HIPK3; TNK2; KAT5; KAT2B; UBE2D2; TPX2; PML; ATN1; PSMD4; PPP1R12A; EGLN2; SH3RF1; EGLN1; DYRK2; PPP1CC; AFF1; DCC; CEB
Enhanced Validation
WB, Y, SPR, Y, YCHAROS
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.)
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.)
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.)
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.)
WB (Western Blot)
(WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate)
WB (Western Blot)
(WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate)
WB (Western Blot)
(Lanes:1: 50ug HEK293T lysate, 2: 50ug A549 lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:5000Gene Name:SIAH2Submitted by:Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena)
WB (Western Blot)
(Lanes:1: 50ug HEK293T lysate, 2: 50ug A549 lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:5000Gene Name:SIAH2Submitted by:Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena)
WB (Western Blot)
(Host: RabbitTarget Name: SIAH2Sample Type: MCF7Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7)
WB (Western Blot)
(Host: RabbitTarget Name: SIAH2Sample Type: MCF7Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7)
WB (Western Blot)
(Host: RabbitTarget Name: SIAH2Sample Type: HepG2Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2)
WB (Western Blot)
(Host: RabbitTarget Name: SIAH2Sample Type: HepG2Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2)
WB (Western Blot)
(Host: RabbitTarget Name: SIAH2Sample Type: HelaLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)
WB (Western Blot)
(Host: RabbitTarget Name: SIAH2Sample Type: HelaLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)
Related Product Information for anti-SIAH2 antibody
This is a rabbit polyclonal antibody against SIAH2. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: SIAH2 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Target Description: SIAH2 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Product Categories/Family for anti-SIAH2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase SIAH2
NCBI Official Synonym Full Names
siah E3 ubiquitin protein ligase 2
NCBI Official Symbol
SIAH2
NCBI Official Synonym Symbols
hSiah2
NCBI Protein Information
E3 ubiquitin-protein ligase SIAH2
UniProt Protein Name
E3 ubiquitin-protein ligase SIAH2
UniProt Gene Name
SIAH2
UniProt Synonym Gene Names
Siah-2; hSiah2
UniProt Entry Name
SIAH2_HUMAN
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Product Notes
The SIAH2 siah2 (Catalog #AAA23577) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIAH2 antibody - N-terminal region reacts with Tested Species Reactivity: Human Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's SIAH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the SIAH2 siah2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: SRPSSTGPSA NKPCSKQPPP QPQHTPSPAA PPAAATISAA GPGSSAVPAA. It is sometimes possible for the material contained within the vial of "SIAH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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