WB (Western Blot)
(Detection of human SIK2 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SIK2 antibody AAA211963 (lot AAA211963-2) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human SIK2 Polyclonal Antibody | anti-SIK2 antibody
Rabbit anti-SIK2 Antibody, Affinity Purified
Gene Names
SIK2; QIK; SNF1LK2; LOH11CR1I
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SIK2, Antibody; Rabbit anti-SIK2 Antibody, Affinity Purified; SIK-2; SNF1-like kinase 2; serine/threonine-protein kinase SNF1-like kinase 2; serine/threonine-protein kinase SIK2; salt-inducible serine/threonine kinase 2; LOH11CR1I; Salt-inducible kinase 2; qin-induced kinase; QIK; salt-inducible protein kinase 2; SNF1LK2; Serine/threonine-protein kinase SIK2; salt inducible kinase 2; anti-SIK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SIK2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 600 and 650
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SIK2 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SIK2 antibody AAA211963 (lot AAA211963-2) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SIK2 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SIK2 antibody AAA211963 (lot AAA211963-2) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SIK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SIK2 antibody AAA211963 (lot AAA211963-2) used for IP at 3 ug per reaction. SIK2 was also immunoprecipitated by rabbit anti-SIK2 antibody For blotting immunoprecipitated SIK2, AAA211963 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SIK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SIK2 antibody AAA211963 (lot AAA211963-2) used for IP at 3 ug per reaction. SIK2 was also immunoprecipitated by rabbit anti-SIK2 antibody For blotting immunoprecipitated SIK2, AAA211963 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SIK2 antibody
Salt-inducible protein kinase 2 (SIK2) phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Sik2 inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H0K1].
Product Categories/Family for anti-SIK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,915 Da
NCBI Official Full Name
serine/threonine-protein kinase SIK2
NCBI Official Synonym Full Names
salt-inducible kinase 2
NCBI Official Symbol
SIK2
NCBI Official Synonym Symbols
QIK; SNF1LK2; LOH11CR1I
NCBI Protein Information
serine/threonine-protein kinase SIK2; SIK-2; SNF1-like kinase 2; qin-induced kinase; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine-protein kinase SNF1-like kinase 2
UniProt Protein Name
Serine/threonine-protein kinase SIK2
UniProt Gene Name
SIK2
UniProt Synonym Gene Names
; SIK-2
UniProt Entry Name
SIK2_HUMAN
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Product Notes
The SIK2 sik2 (Catalog #AAA211963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SIK2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIK2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SIK2 sik2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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