WB (Western Blot)
(Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Rabbit SKA1 Polyclonal Antibody | anti-SKA1 antibody
SKA1 Antibody
Gene Names
SKA1; C18orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
SKA1 Antibody is affinity chromatography purified via peptide column.
Synonyms
SKA1, Antibody; SKA1 Antibody; SKA1; C18orf24; Spindle and kinetochore-associated protein 1; spindle and kinetochore associated complex subunit 1; anti-SKA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SKA1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; SKA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
255
Applicable Applications for anti-SKA1 antibody
WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
SKA1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA1. The immunogen is located within amino acids 180 - 230 of SKA1.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Preparation and Storage
SKA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
WB (Western Blot)
(Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.)
WB (Western Blot)
(Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Related Product Information for anti-SKA1 antibody
SKA1 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,390 Da
NCBI Official Full Name
Spindle and kinetochore-associated protein 1
NCBI Official Synonym Full Names
spindle and kinetochore associated complex subunit 1
NCBI Official Symbol
SKA1
NCBI Official Synonym Symbols
C18orf24
NCBI Protein Information
spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1
UniProt Protein Name
Spindle and kinetochore-associated protein 1
UniProt Gene Name
SKA1
UniProt Synonym Gene Names
C18orf24
UniProt Entry Name
SKA1_HUMAN
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Product Notes
The SKA1 ska1 (Catalog #AAA39242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SKA1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SKA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SKA1 ska1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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