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Application Data
(AAA285612 staining SLC16A10 in Humanskeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as thesecondary antibody)
Rabbit anti-Human SLC16A10 Polyclonal Antibody | anti-SLC16A10 antibody
SLC16A10 Antibody (C-term)
Gene Names
SLC16A10; TAT1; MCT10; PRO0813
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SLC16A10, Antibody; SLC16A10 Antibody (C-term); Monocarboxylate transporter 10; MCT 10; Aromatic amino acid transporter 1; Solute carrier family 16 member 10; T-type amino acid transporter 1; SLC16A10; MCT10; TAT1; anti-SLC16A10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SLC16A10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-329 amino acids from the C-terminal region of human SLC16A10.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
301-329
Sequence Length
515
Applicable Applications for anti-SLC16A10 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Sodium-independent transporter thatmediates the uptake of aromatic acids. Canfunction as a net efflux pathway for aromaticamino acids in the basosolateral epithelialcells (By similarity)
Cellular location
Cell membrane; Multi-pass membraneprotein. Basolateral cell membrane
Tissue location
Strongly expressed in kidney and skeletalmuscle and at lower level in placenta andheart
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks.For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application Data
(AAA285612 staining SLC16A10 in Humanskeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as thesecondary antibody)
Application Data
(AAA285612 staining SLC16A10 in Humanskeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as thesecondary antibody)
FCM/FACS (Flow Cytometry)
(SLC16A10 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SLC16A10 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(SLC16A10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC16A10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(SLC16A10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC16A10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SLC16A10 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).)
WB (Western Blot)
(SLC16A10 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).)
WB (Western Blot)
(All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: CCRF-CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: CCRF-CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SLC16A10 antibody
SLC16A10 is a member of a family of plasma membrane amino
acid transporters that mediate the Na(+)-independent transport of
aromatic amino acids across the plasma membrane.
acid transporters that mediate the Na(+)-independent transport of
aromatic amino acids across the plasma membrane.
Product Categories/Family for anti-SLC16A10 antibody
References
Loubiere, L.S., et al. Placenta 31(4):295-304(2010)
Friesema, E.C., et al. Mol. Endocrinol. 22(6):1357-1369(2008)
Broer, S. Physiol. Rev. 88(1):249-286(2008)
Park, S.Y., et al. Arch. Pharm. Res. 28(4):421-432(2005)
Halestrap, A.P., et al. Pflugers Arch. 447(5):619-628(2004)
Friesema, E.C., et al. Mol. Endocrinol. 22(6):1357-1369(2008)
Broer, S. Physiol. Rev. 88(1):249-286(2008)
Park, S.Y., et al. Arch. Pharm. Res. 28(4):421-432(2005)
Halestrap, A.P., et al. Pflugers Arch. 447(5):619-628(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
monocarboxylate transporter 10
NCBI Official Synonym Full Names
solute carrier family 16 (aromatic amino acid transporter), member 10
NCBI Official Symbol
SLC16A10
NCBI Official Synonym Symbols
TAT1; MCT10; PRO0813
NCBI Protein Information
monocarboxylate transporter 10
UniProt Protein Name
Monocarboxylate transporter 10
UniProt Gene Name
SLC16A10
UniProt Synonym Gene Names
MCT10; TAT1; MCT 10
UniProt Entry Name
MOT10_HUMAN
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Product Notes
The SLC16A10 slc16a10 (Catalog #AAA285612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 301-329. The SLC16A10 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC16A10 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SLC16A10 slc16a10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC16A10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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