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IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA26999 at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Rabbit anti-Human, Mouse SLC7A6 Polyclonal Antibody | anti-SLC7A6 antibody
SLC7A6 Antibody
Gene Names
SLC7A6; LAT3; LAT-2; y+LAT-2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
SLC7A6, Antibody; SLC7A6 Antibody; Y+L amino acid transporter 2; Cationic amino acid transporter; y+ system; Solute carrier family 7 member 6; y(+)L-type amino acid transporter 2; Y+LAT2; y+LAT-2; SLC7A6, KIAA0245; anti-SLC7A6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Applicable Applications for anti-SLC7A6 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Recombinant Human Y+L amino acid transporter 2 protein (473-515AA)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Immuogen Species
Human
Research Area
Neuroscience
Target Names
SLC7A6
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA26999 at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA26999 at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IHC (Immunohistochemistry)
(IHC image of AAA26999 diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA26999 diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA26999 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA26999 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, Mouse Heart tissue lysateAll lanes: SLC7A6 antibody at 1:1000Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57 kDaObserved band size: 57 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, Mouse Heart tissue lysateAll lanes: SLC7A6 antibody at 1:1000Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57 kDaObserved band size: 57 kDa)
Related Product Information for anti-SLC7A6 antibody
Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Also acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L-arginine in monocytes. Reduces uptake of ornithine in retinal pigment epithelial (RPE) cells.
Product Categories/Family for anti-SLC7A6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Y+L amino acid transporter 2
NCBI Official Synonym Full Names
solute carrier family 7 member 6
NCBI Official Symbol
SLC7A6
NCBI Official Synonym Symbols
LAT3; LAT-2; y+LAT-2
NCBI Protein Information
Y+L amino acid transporter 2
UniProt Protein Name
Y+L amino acid transporter 2
UniProt Gene Name
SLC7A6
UniProt Synonym Gene Names
Y+LAT2; y+LAT-2
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Product Notes
The SLC7A6 slc7a6 (Catalog #AAA26999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC7A6 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC7A6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the SLC7A6 slc7a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC7A6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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