Rabbit anti-Human SLC9A3R1 Polyclonal Antibody | anti-SLC9A3R1 antibody

Anti-SLC9A3R1 Antibody, Rabbit Polyclonal

Cat. Num. AAA256054

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: SLC9A3R1, Antibody; Anti-SLC9A3R1 Antibody, Rabbit Polyclonal; SLC9A3R1 Antibody, Rabbit PAb, Antigen Affinity Purified; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; Anti-EBP50 Antibody; Anti-NHERF Antibody; Anti-NHERF-1 Antibody; Anti-NHERF1 Antibody; Anti-NPHLOP2 Antibody; anti-SLC9A3R1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SLC9A3R1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SLC9A3R1 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human SLC9A3R1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human SLC9A3R1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(SLC9A3R1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-SLC9A3R1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SLC9A3R1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size :50 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human SLC9A3R1 in human epididymis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human SLC9A3R1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-SLC9A3R1 rabbit polyclonal antibody at 1:2000 dilutionLane A: HepG2 Whole Cell LysateLane B: Caco 2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:50 kDa)

Related Product Information for anti-SLC9A3R1 antibody

SLC9A3R1 (solute carrier family 9, subfamily A [NHE3, cation proton antiporter 3], member 3 regulator 1) is involved in suppressing breast cancer cell proliferation and the SLC9A3R1-related signaling pathway regulates the activation of autophagy processes. Na+/H+ exchanger regulatory factor-1 (NHERF1), encoded by the Slc9a3r1 gene, functions as a scaffold protein, which is implicated in the regulation of membrane expression of various cell-surface proteins. SLC9A3R1 is a PDZ domain-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family and is implicated in diverse aspects of epithelial membrane biology and immune synapse formation in T cells. The SLC9A3R1-BECN1 signaling pathway participates in the activation of autophagy processes in breast cancer cells. Defective regulation of SLC9A3R1 or NAT9 by RUNX1 as a susceptibility factor for psoriasis.

References

Tsurudome Y, et al. (2018) Circadian clock component period2 regulates diurnal expression of na(+)/h(+) exchanger regulatory factor-1 and its scaffolding function. Sci Rep 8 (1): 9072.Liu H, et al. (2015) Slc9a3r1 stimulates autophagy via becn1 stabilization in breast cancer cells. Autophagy 11 (12): 2323-2334.Helms C, et al. (2003) A putative runx1 binding site variant between slc9a3r1 and nat9 is associated with susceptibility to psoriasis. Nat Genet 35 (4): 349-356.