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FCM/FACS (Flow Cytometry)
(SMAD3 Antibody (S213) (Cat. #AAA297738) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Dilution: FC 1:10~50)
Rabbit SMAD3 Polyclonal Antibody | anti-SMAD3 antibody
SMAD3 Antibody (S213)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
HumanPredicted: Chicken, Mouse, Pig, Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SMAD3, Antibody; SMAD3 Antibody (S213); Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; RB29526; anti-SMAD3 antibody
Host
Rabbit
Reactivity
Human
Predicted: Chicken, Mouse, Pig, Rat
Predicted: Chicken, Mouse, Pig, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-220 amino acids from human SMAD3.
Purity/Purification
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-SMAD3 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot)
Antigen Source
Human
Antigen Region
191-220
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(SMAD3 Antibody (S213) (Cat. #AAA297738) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Dilution: FC 1:10~50)
FCM/FACS (Flow Cytometry)
(SMAD3 Antibody (S213) (Cat. #AAA297738) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Dilution: FC 1:10~50)
IHC (Immunohistochemistry)
(SMAD3 Antibody (S213) (Cat. #AAA297738)immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMAD3 Antibody (S213) for immunohistochemistry. Clinical relevance has not been evaluated.Dilution: IHC-P 1:10~50)
IHC (Immunohistochemistry)
(SMAD3 Antibody (S213) (Cat. #AAA297738)immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMAD3 Antibody (S213) for immunohistochemistry. Clinical relevance has not been evaluated.Dilution: IHC-P 1:10~50)
WB (Western Blot)
(SMAD3 Antibody (S213) (Cat. #AAA297738) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).Dilution: WB 1:1000)
WB (Western Blot)
(SMAD3 Antibody (S213) (Cat. #AAA297738) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).Dilution: WB 1:1000)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SMAD3 Antibody (S213) (Cat#AAA297738) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Dilution: IF 1:10~50)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SMAD3 Antibody (S213) (Cat#AAA297738) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Dilution: IF 1:10~50)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SMAD3 Antibody (S213) (Cat#AAA297738) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Dilution: IF 1:10~50)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SMAD3 Antibody (S213) (Cat#AAA297738) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Dilution: IF 1:10~50)
Related Product Information for anti-SMAD3 antibody
The protein encoded by this gene belongs to the SMAD, afamily of proteins similar to the gene products of the Drosophilagene 'mothers against decapentaplegic' (Mad) and the C. elegansgene Sma. SMAD proteins are signal transducers and transcriptionalmodulators that mediate multiple signaling pathways. This proteinfunctions as a transcriptional modulator activated by transforminggrowth factor-beta and is thought to play a role in the regulationof carcinogenesis.
Product Categories/Family for anti-SMAD3 antibody
References
Ge, Q., et al. J. Cell. Physiol. 225 (3):846-854 (2010)Lee, J., et al. J. Biol. Chem. 285 (34):26618-26627 (2010)Roder, C., et al. Childs Nerv Syst (2010) In press :Valdes, A.M., et al. Arthritis Rheum. 62 (8):2347-2352 (2010)Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,081 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 2
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
NCBI Protein Information
mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentapleg
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
UniProt Entry Name
SMAD3_HUMAN
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Product Notes
The SMAD3 smad3 (Catalog #AAA297738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMAD3 Antibody (S213) reacts with Human Predicted: Chicken, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SMAD3 smad3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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