WB (Western Blot)
(SMAD4 antibody (pAb) tested by Western blot. Detection of SMAD4 by Western blot analysis. Nuclear extract of HeLa cells (30 ug) probed with SMAD4 antibody at a dilution of 1:500.)
Rabbit anti-Human SMAD4 Polyclonal Antibody | anti-SMAD4 antibody
SMAD4 antibody (pAb)
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
SMAD4, Antibody; SMAD4 antibody (pAb); JIP; DPC4; MADH4; MYHRS; anti-SMAD4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-SMAD4 antibody
WB (Western Blot), IP (Immunoprecipitation)
Immunogen
This antibody was raised against a peptide within the N-terminal region of human SMAD4.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(SMAD4 antibody (pAb) tested by Western blot. Detection of SMAD4 by Western blot analysis. Nuclear extract of HeLa cells (30 ug) probed with SMAD4 antibody at a dilution of 1:500.)
WB (Western Blot)
(SMAD4 antibody (pAb) tested by Western blot. Detection of SMAD4 by Western blot analysis. Nuclear extract of HeLa cells (30 ug) probed with SMAD4 antibody at a dilution of 1:500.)
WB (Western Blot)
(SMAD4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SMAD4 antibody was used to immunoprecipitate SMAD4 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMAD4 antibody at a dilution of 1:500.)
WB (Western Blot)
(SMAD4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SMAD4 antibody was used to immunoprecipitate SMAD4 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMAD4 antibody at a dilution of 1:500.)
Related Product Information for anti-SMAD4 antibody
Short Description: Our SMAD4 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: SMAD4 (Mothers Against Decapentaplegic Homolog 4) or common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Background: SMAD4 (Mothers Against Decapentaplegic Homolog 4) or common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Product Categories/Family for anti-SMAD4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62.6 kDa (572 aa)
NCBI Official Full Name
mothers against decapentaplegic homolog 4
NCBI Official Synonym Full Names
SMAD family member 4
NCBI Official Symbol
SMAD4
NCBI Official Synonym Symbols
JIP; DPC4; MADH4; MYHRS
NCBI Protein Information
mothers against decapentaplegic homolog 4
UniProt Protein Name
Mothers against decapentaplegic homolog 4
UniProt Gene Name
SMAD4
UniProt Synonym Gene Names
DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
UniProt Entry Name
SMAD4_HUMAN
Similar Products
Product Notes
The SMAD4 smad4 (Catalog #AAA60063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMAD4 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the SMAD4 smad4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.