FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-SNAP25 antibody (AAA127072).Overlay histogram showing SH-SY5Y cells stained with AAA127072 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (AAA127072, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit SNAP25 Polyclonal Antibody | anti-SNAP25 antibody
Anti-SNAP25 Antibody Picoband
Gene Names
SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
SNAP25, Antibody; Anti-SNAP25 Antibody Picoband; Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody; anti-SNAP25 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Expressed in all tissues.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-SNAP25 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
E coli-derived human SNAP25 recombinant protein (Position: R31-L203). Human SNAP25 shares 100% amino acid (aa) sequence identity with both mouse and rat SNAP25.
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-SNAP25 antibody (AAA127072).Overlay histogram showing SH-SY5Y cells stained with AAA127072 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (AAA127072, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-SNAP25 antibody (AAA127072).Overlay histogram showing SH-SY5Y cells stained with AAA127072 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (AAA127072, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of SNAP25 using anti-SNAP25 antibody (AAA127072).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (#AAA127072) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 24 kDa. The expected band size for SNAP25 is at 24 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of SNAP25 using anti-SNAP25 antibody (AAA127072).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (#AAA127072) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 24 kDa. The expected band size for SNAP25 is at 24 kDa.)
Related Product Information for anti-SNAP25 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-SNAP25 antibody
References
1. An, S. J., Almers, W. Tracking SNARE complex formation in live endocrine cells. Science 306: 1042-1046, 2004. 2. Bark, I. C., Wilson, M. C. Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25. Gene 139: 291-292, 1994.3. Barr, C. L., Feng, Y., Wigg, K., Bloom, S., Roberts, W., Malone, M., Schachar, R., Tannock, R., Kennedy, J. L. Identification of DNA variants in the SNAP-25 gene and linkage study of these polymorphisms and attention-deficit hyperactivity disorder. Molec. Psychiat. 5: 405-409, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,315 Da
NCBI Official Full Name
synaptosomal-associated protein 25 isoform SNAP25A
NCBI Official Synonym Full Names
synaptosomal-associated protein, 25kDa
NCBI Official Symbol
SNAP25
NCBI Official Synonym Symbols
RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
NCBI Protein Information
synaptosomal-associated protein 25; SUP; super protein; resistance to inhibitors of cholinesterase 4 homolog
UniProt Protein Name
Synaptosomal-associated protein 25
UniProt Gene Name
SNAP25
UniProt Synonym Gene Names
SNAP; SNAP-25; SUP
UniProt Entry Name
SNP25_HUMAN
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Product Notes
The SNAP25 snap25 (Catalog #AAA127072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SNAP25 Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP25 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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