WB (Western Blot)
(Western blot analysis of SNF8 (arrow) using rabbit polyclonal SNF8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.)
Rabbit SNF8 Polyclonal Antibody | anti-SNF8 antibody
SNF8 Antibody (N-term)
Gene Names
SNF8; Dot3; EAP30; VPS22
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SNF8, Antibody; SNF8 Antibody (N-term); Vacuolar-sorting protein SNF8; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22; SNF8; EAP30; anti-SNF8 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human SNF8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
37-65
Sequence Length
257
Applicable Applications for anti-SNF8 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of SNF8 (arrow) using rabbit polyclonal SNF8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.)
WB (Western Blot)
(Western blot analysis of SNF8 (arrow) using rabbit polyclonal SNF8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.)
WB (Western Blot)
(SNF8 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SNF8 antibody detected the SNF8 protein (arrow).)
WB (Western Blot)
(SNF8 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SNF8 antibody detected the SNF8 protein (arrow).)
Related Product Information for anti-SNF8 antibody
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form
ESCRT-II (endosomal sorting complex required for transport II), a
complex involved in endocytosis of ubiquitinated membrane proteins.
SNF8, VPS25, and VPS36 are also associated in a multiprotein
complex with RNA polymerase II elongation factor (ELL; MIM 600284)
(Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001
[PubMed 11278625]).
ESCRT-II (endosomal sorting complex required for transport II), a
complex involved in endocytosis of ubiquitinated membrane proteins.
SNF8, VPS25, and VPS36 are also associated in a multiprotein
complex with RNA polymerase II elongation factor (ELL; MIM 600284)
(Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001
[PubMed 11278625]).
References
Malerod, L., et al. Traffic 8(11):1617-1629(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Langelier, C., et al. J. Virol. 80(19):9465-9480(2006)
Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006)
Slagsvold, T., et al. J. Biol. Chem. 280(20):19600-19606(2005)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Langelier, C., et al. J. Virol. 80(19):9465-9480(2006)
Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006)
Slagsvold, T., et al. J. Biol. Chem. 280(20):19600-19606(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28864
NCBI Official Full Name
vacuolar-sorting protein SNF8
NCBI Official Synonym Full Names
SNF8, ESCRT-II complex subunit
NCBI Official Symbol
SNF8
NCBI Official Synonym Symbols
Dot3; EAP30; VPS22
NCBI Protein Information
vacuolar-sorting protein SNF8
UniProt Protein Name
Vacuolar-sorting protein SNF8
UniProt Gene Name
SNF8
UniProt Synonym Gene Names
EAP30; hVps22
UniProt Entry Name
SNF8_HUMAN
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Product Notes
The SNF8 snf8 (Catalog #AAA285478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 37-65. The SNF8 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SNF8 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SNF8 snf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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